Design of amperometric urea biosensor based on self-assembled monolayer of cystamine/PAMAM-grafted MWCNT/Urease

Muamer Dervisevic, Esma Dervisevic, Mehmet Şenel

Research output: Contribution to journalArticleResearchpeer-review

44 Citations (Scopus)

Abstract

In this work, we report novel amperometric urea biosensor based on immobilization of Urease enzyme onto self-assembled polyamidoamine grafted multiwalled carbon nanotube (MWCNT-PAMAM) dendrimers (from G1, first generation up to G5) on Au electrode. Bio-sensing electrode's performance parameters such as optimum pH, temperature, and applied potential together with reproducibility were investigated. The electrode based on fifth generation of MWCNT-PAMAM and urease enzyme showed excellent performance in urea detection at an applied potential of 0.45 V with short response time of 3 s, wide linear range of 1–20 mM, detection limit of 0.4 mM, and sensitivity of 6.6 nA/mM. No significant response was detected in the presence of possible interferon molecules. Non-diluted human blood plasma has been used for real sample application where electrode showed excellent performance in urea detection.

Original languageEnglish
Pages (from-to)93-101
Number of pages9
JournalSensors and Actuators B: Chemical
Volume254
DOIs
Publication statusPublished - Jan 2018
Externally publishedYes

Keywords

  • Amperometric biosensor
  • MWCNT
  • PAMAM dendrimers
  • Urea
  • Urease

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