Description of a mouse monoclonal anti-HLA-B27 antibody HLA-ABC-m3

Joseph A. Trapani; Hilary A. Vaughan; Rosemary L. Sparrow; Brian D. Tait; Ian F.C. McKenzie

doi:10.1016/0198-8859(83)90058-7

Description of a mouse monoclonal anti-HLA-B27 antibody HLA-ABC-m3

Joseph A. Trapani, Hilary A. Vaughan, Rosemary L. Sparrow, Brian D. Tait, Ian F.C. McKenzie

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Abstract

The production and characterization of a new anti-HLA-B27 monoclonal antibody HLA-ABC-m3 is described. This cytotoxic IgG2a antibody binds protein A and is able to precipitate cell surface molecules of 43,000 and 12,000 daltons corresponding to the HLA heavy chain and β2-microglobulin. Population testing revealed that the HLA-ABC-m3 antibody reacted with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27⁺ and with 5/105 HLA-B27^- individuals. These five extra reactions were with individuals expressing the cross-reactive HLA-B7 alloantigen, although the affinity of the monoclonal antibody for B27 heterozygous individuals (approx 10⁹ M^-1) was tenfold greater than with B7 individuals (approx 10⁸ M^-1). In addition, HLA-ABC-m3 reactivity segregated with HLA-B27 in two families. This monoclonal antibody should be of value in the investigation of the role of HLA-B27 in disease.

Original language	English
Pages (from-to)	205-216
Number of pages	12
Journal	Human Immunology
Volume	7
Issue number	4
DOIs	https://doi.org/10.1016/0198-8859(83)90058-7
Publication status	Published - Aug 1983
Externally published	Yes

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title = "Description of a mouse monoclonal anti-HLA-B27 antibody HLA-ABC-m3",

abstract = "The production and characterization of a new anti-HLA-B27 monoclonal antibody HLA-ABC-m3 is described. This cytotoxic IgG2a antibody binds protein A and is able to precipitate cell surface molecules of 43,000 and 12,000 daltons corresponding to the HLA heavy chain and β2-microglobulin. Population testing revealed that the HLA-ABC-m3 antibody reacted with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27+ and with 5/105 HLA-B27- individuals. These five extra reactions were with individuals expressing the cross-reactive HLA-B7 alloantigen, although the affinity of the monoclonal antibody for B27 heterozygous individuals (approx 109 M-1) was tenfold greater than with B7 individuals (approx 108 M-1). In addition, HLA-ABC-m3 reactivity segregated with HLA-B27 in two families. This monoclonal antibody should be of value in the investigation of the role of HLA-B27 in disease.",

author = "Trapani, {Joseph A.} and Vaughan, {Hilary A.} and Sparrow, {Rosemary L.} and Tait, {Brian D.} and McKenzie, {Ian F.C.}",

note = "Funding Information: ACKNOWLEDGMENT Grant support for the studies was obtained from the National Health and Medical Research Council of Australia. It is proposed to distribute this antibody through Australian Monoclonal Development, Sydney, Australia. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1983",

month = aug,

doi = "10.1016/0198-8859(83)90058-7",

language = "English",

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pages = "205--216",

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T1 - Description of a mouse monoclonal anti-HLA-B27 antibody HLA-ABC-m3

AU - Trapani, Joseph A.

AU - Vaughan, Hilary A.

AU - Sparrow, Rosemary L.

AU - Tait, Brian D.

AU - McKenzie, Ian F.C.

N1 - Funding Information: ACKNOWLEDGMENT Grant support for the studies was obtained from the National Health and Medical Research Council of Australia. It is proposed to distribute this antibody through Australian Monoclonal Development, Sydney, Australia. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1983/8

Y1 - 1983/8

N2 - The production and characterization of a new anti-HLA-B27 monoclonal antibody HLA-ABC-m3 is described. This cytotoxic IgG2a antibody binds protein A and is able to precipitate cell surface molecules of 43,000 and 12,000 daltons corresponding to the HLA heavy chain and β2-microglobulin. Population testing revealed that the HLA-ABC-m3 antibody reacted with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27+ and with 5/105 HLA-B27- individuals. These five extra reactions were with individuals expressing the cross-reactive HLA-B7 alloantigen, although the affinity of the monoclonal antibody for B27 heterozygous individuals (approx 109 M-1) was tenfold greater than with B7 individuals (approx 108 M-1). In addition, HLA-ABC-m3 reactivity segregated with HLA-B27 in two families. This monoclonal antibody should be of value in the investigation of the role of HLA-B27 in disease.

AB - The production and characterization of a new anti-HLA-B27 monoclonal antibody HLA-ABC-m3 is described. This cytotoxic IgG2a antibody binds protein A and is able to precipitate cell surface molecules of 43,000 and 12,000 daltons corresponding to the HLA heavy chain and β2-microglobulin. Population testing revealed that the HLA-ABC-m3 antibody reacted with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27+ and with 5/105 HLA-B27- individuals. These five extra reactions were with individuals expressing the cross-reactive HLA-B7 alloantigen, although the affinity of the monoclonal antibody for B27 heterozygous individuals (approx 109 M-1) was tenfold greater than with B7 individuals (approx 108 M-1). In addition, HLA-ABC-m3 reactivity segregated with HLA-B27 in two families. This monoclonal antibody should be of value in the investigation of the role of HLA-B27 in disease.

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JO - Human Immunology

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ER -

Description of a mouse monoclonal anti-HLA-B27 antibody HLA-ABC-m3

Abstract

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