TY - JOUR
T1 - Desalting of peptides and protein mixtures by RP-HPLC techniques
AU - Boysen, Reinhard
AU - Hearn, Milton Thomas William
PY - 2006
Y1 - 2006
N2 - The RP-HPLC technique can be used to a??desalta?? peptide or protein samples derived from extraction procedures, from chemical reactions such as reductive alkylation in the presence of urea or guanidine hydrochloride, citraconylation, iodination, or cyanogen bromide cleavage, or recovered from other chromatographic separation. The peptide or protein solution is injected onto a small RP-HPLC column. An aqueous buffer is used to elute the salts while the peptides or proteins are concentrated at the top of the column. After elution of the salts, monitored by UV detection, the peptides or proteins are eluted with H2O-acetonitrile or H2O-isopropanol mobile phases.
AB - The RP-HPLC technique can be used to a??desalta?? peptide or protein samples derived from extraction procedures, from chemical reactions such as reductive alkylation in the presence of urea or guanidine hydrochloride, citraconylation, iodination, or cyanogen bromide cleavage, or recovered from other chromatographic separation. The peptide or protein solution is injected onto a small RP-HPLC column. An aqueous buffer is used to elute the salts while the peptides or proteins are concentrated at the top of the column. After elution of the salts, monitored by UV detection, the peptides or proteins are eluted with H2O-acetonitrile or H2O-isopropanol mobile phases.
UR - http://cshprotocols.cshlp.org/content/2006/5/pdb.prot4549.full
U2 - 10.1101/pdb.prot4549
DO - 10.1101/pdb.prot4549
M3 - Article
SN - 1559-6095
VL - 5
SP - 1
EP - 6
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
ER -