TY - CHAP
T1 - Deriving and propagating mouse embryonic stem cell lines for studying genomic imprinting
AU - Mann, Jeffrey R.
PY - 2001
Y1 - 2001
N2 - Embryonic stem (ES) cells are a cell culture derivative of the blastocyst inner cell mass (ICM), the latter giving rise to the embryo, the amnion, the yolk sac, and the chrorioallantoic portion of the placenta. Blastocyst injection chimera experiments show that ES cells are similar to early-stage ICM cells in that they contribute to the primitive ectoderm and endoderm derivatives (1). However, it is probably not possible to equate these two cell types, as ES cells appear to be produced by the cell culture environment and have no exact counterpart in the blastocyst. Instead, ES cells could be thought of as being ICM cells that, instead of undergoing rapid differentiation as they would in vivo, are abnormally locked into continuing cycles of division in the undifferentiated state by virtue of the action of exogenous factors. Leukemia inhibitory factor, LIF, is one such factor (2,3) and is indispensable for the propagation of mouse ES cells at least when primary embryo fibroblasts (PEFs) are used as feeder layers (4).
AB - Embryonic stem (ES) cells are a cell culture derivative of the blastocyst inner cell mass (ICM), the latter giving rise to the embryo, the amnion, the yolk sac, and the chrorioallantoic portion of the placenta. Blastocyst injection chimera experiments show that ES cells are similar to early-stage ICM cells in that they contribute to the primitive ectoderm and endoderm derivatives (1). However, it is probably not possible to equate these two cell types, as ES cells appear to be produced by the cell culture environment and have no exact counterpart in the blastocyst. Instead, ES cells could be thought of as being ICM cells that, instead of undergoing rapid differentiation as they would in vivo, are abnormally locked into continuing cycles of division in the undifferentiated state by virtue of the action of exogenous factors. Leukemia inhibitory factor, LIF, is one such factor (2,3) and is indispensable for the propagation of mouse ES cells at least when primary embryo fibroblasts (PEFs) are used as feeder layers (4).
UR - http://www.scopus.com/inward/record.url?scp=33644478322&partnerID=8YFLogxK
U2 - 10.1385/1-59259-211-2:21
DO - 10.1385/1-59259-211-2:21
M3 - Chapter (Book)
C2 - 12843439
AN - SCOPUS:33644478322
SN - 9780896037410
SN - 9781617371646
VL - 181
T3 - Methods in Molecular Biology
SP - 21
EP - 39
BT - Genomic Imprinting
PB - Humana Press
CY - USA
ER -