Abstract
We describe derivation of induced pluripotent stem cells (iPSCs) from terminally differentiated mouse cells in serum-and feeder-free stirred suspension cultures. Temporal analysis of global gene expression revealed high correlations between cells reprogrammed in suspension and cells reprogrammed in adhesion-dependent conditions. Suspension culture-reprogrammed iPSCs (SiPSCs) could be differentiated into all three germ layers in vitro and contributed to chimeric embryos in vivo. SiPSC generation allowed for efficient selection of reprogramming factor-expressing cells based on their differential survival and proliferation in suspension culture. Seamless integration of SiPSC reprogramming and directed differentiation enabled scalable production of beating cardiac cells in a continuous single cell-and small aggregate-based process. This method is an important step toward the development of robust PSC generation, expansion and differentiation technology.
Original language | English |
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Pages (from-to) | 509-516 |
Number of pages | 8 |
Journal | Nature Methods |
Volume | 9 |
Issue number | 5 |
DOIs | |
Publication status | Published - May 2012 |
Externally published | Yes |