Projects per year
Abstract
Eggs contain about 200,000 mitochondria that generate adenosine triphosphate and metabolites essential for oocyte development. Mitochondria also integrate metabolism and transcription via metabolites that regulate epigenetic modifiers, but there is no direct evidence linking oocyte mitochondrial function to the maternal epigenome and subsequent embryo development. Here, we have disrupted oocyte mitochondrial function via deletion of the mitochondrial fission factor Drp1. Fission-deficient oocytes exhibit a high frequency of failure in peri- and postimplantation development. This is associated with altered mitochondrial function, changes in the oocyte transcriptome and proteome, altered subcortical maternal complex, and a decrease in oocyte DNA methylation and H3K27me3. Transplanting pronuclei of fertilized Drp1 knockout oocytes to normal ooplasm fails to rescue embryonic lethality. We conclude that mitochondrial function plays a role in establishing the maternal epigenome, with serious consequences for embryo development.
Original language | English |
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Article number | eabl8070 |
Number of pages | 15 |
Journal | Science Advances |
Volume | 8 |
Issue number | 24 |
DOIs | |
Publication status | Published - Jun 2022 |
Projects
- 2 Finished
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Mitochondrial metabolism and its role in the maternal epigenome.
Carroll, J. (Primary Chief Investigator (PCI)) & Adhikari, D. (Chief Investigator (CI))
1/01/19 → 31/12/22
Project: Research
Equipment
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Bioinformatics Platform
Deveson, D. (Manager)
Faculty of Medicine Nursing and Health Sciences Research PlatformsFacility/equipment: Facility
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Histology Platform
Cohen, C. (Manager)
Faculty of Medicine Nursing and Health Sciences Research PlatformsFacility/equipment: Facility
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Monash Micro Imaging
Firth, S. (Manager), Fulcher, A. (Operator), Chernyavskiy, O. (Operator), Rzeszutek, M. (Other), Potter, D. (Manager), Hilsenstein, V. (Operator), Nunez-Iglesias, J. (Other), Cody, S. (Manager), Carmichael, I. (Operator), Kouskousis, B. (Other), Creed, S. (Manager) & Ballerin, G. (Operator)
Faculty of Medicine Nursing and Health Sciences Research PlatformsFacility/equipment: Facility