TY - JOUR
T1 - Dengue virus population genetics in Yogyakarta, Indonesia prior to city-wide Wolbachia deployment
AU - Arguni, Eggi
AU - Indriani, Citra
AU - Rahayu, Ayu
AU - Supriyati, Endah
AU - Yohan, Benediktus
AU - Hayati, Rahma F.
AU - Wardana, Satrio
AU - Tantowijoyo, Warsito
AU - Anshari, Muhammad Ridwan
AU - Rahayu, Endang
AU - Rubangi, null
AU - Ahmad, Riris Andono
AU - Utarini, Adi
AU - Simmons, Cameron P.
AU - Sasmono, R. Tedjo
N1 - Funding Information:
The Tahija Foundation provided the financial support for World Mosquito Program activities in Indonesia. The Wellcome Trust and Bill and Melinda Gates Foundation provide financial support to the World Mosquito Program.
Publisher Copyright:
© 2022 The Authors
PY - 2022/8
Y1 - 2022/8
N2 - Dengue has been endemic in Yogyakarta, Indonesia for decades. Here, we report the dengue epidemiology, entomology, and virology in Yogyakarta in 2016–2017, prior to the commencement of the Applying Wolbachia to Eliminate Dengue (AWED) randomized trial. Dengue epidemiological data were compiled and blood samples from dengue-suspected patients were tested for dengue virus (DENV). Ae. aegypti mosquito samples were caught from the field using BG-Sentinel traps and tested for the presence of DENV infection. Sequencing of the DENV E gene was used to determine the phylogeny and genotypes of circulating DENV. Within the last decade, the 2016–2017 dengue incidence was considered very high. Among the 649 plasma samples collected between March 2016–February 2017; and 36,910 mosquito samples collected between December 2016–May 2017, a total of 197 and 38 samples were DENV-positive by qRT-PCR, respectively. All four DENV serotypes were detected, with DENV-3 (n = 88; 44.67%) and DENV-1 (n = 87; 44.16%) as the predominant serotype, followed by DENV-4 (n = 12; 6.09%) and DENV-2 (n = 10; 5.08%). The Yogyakarta DENV-1 isolates were classified into Genotype I and IV, while DENV-2, DENV-3, and DENV-4 isolates were classified into the Cosmopolitan genotype, Genotype I, and Genotype II, respectively. Yogyakarta DENV isolates were closely related to Indonesian strains from neighboring Javanese cities, consistent with the endemic circulation of DENV on this highly populous island. Our study provides comprehensive baseline information on the DENV population genetic characteristics in Yogyakarta, which are useful as baseline data for the AWED trial and the future DENV surveillance in the city in the presence of a Wolbachia-infected Ae. aegypti population.
AB - Dengue has been endemic in Yogyakarta, Indonesia for decades. Here, we report the dengue epidemiology, entomology, and virology in Yogyakarta in 2016–2017, prior to the commencement of the Applying Wolbachia to Eliminate Dengue (AWED) randomized trial. Dengue epidemiological data were compiled and blood samples from dengue-suspected patients were tested for dengue virus (DENV). Ae. aegypti mosquito samples were caught from the field using BG-Sentinel traps and tested for the presence of DENV infection. Sequencing of the DENV E gene was used to determine the phylogeny and genotypes of circulating DENV. Within the last decade, the 2016–2017 dengue incidence was considered very high. Among the 649 plasma samples collected between March 2016–February 2017; and 36,910 mosquito samples collected between December 2016–May 2017, a total of 197 and 38 samples were DENV-positive by qRT-PCR, respectively. All four DENV serotypes were detected, with DENV-3 (n = 88; 44.67%) and DENV-1 (n = 87; 44.16%) as the predominant serotype, followed by DENV-4 (n = 12; 6.09%) and DENV-2 (n = 10; 5.08%). The Yogyakarta DENV-1 isolates were classified into Genotype I and IV, while DENV-2, DENV-3, and DENV-4 isolates were classified into the Cosmopolitan genotype, Genotype I, and Genotype II, respectively. Yogyakarta DENV isolates were closely related to Indonesian strains from neighboring Javanese cities, consistent with the endemic circulation of DENV on this highly populous island. Our study provides comprehensive baseline information on the DENV population genetic characteristics in Yogyakarta, which are useful as baseline data for the AWED trial and the future DENV surveillance in the city in the presence of a Wolbachia-infected Ae. aegypti population.
KW - Dengue
KW - Epidemiology
KW - Genotype
KW - Phylogeny
KW - Serotype
KW - Wolbachia
KW - Yogyakarta
UR - http://www.scopus.com/inward/record.url?scp=85132372552&partnerID=8YFLogxK
U2 - 10.1016/j.meegid.2022.105308
DO - 10.1016/j.meegid.2022.105308
M3 - Article
C2 - 35644356
AN - SCOPUS:85132372552
VL - 102
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
SN - 1567-1348
M1 - 105308
ER -
