TY - JOUR
T1 - Dendritic cell-dependent in vivo generation of autoregulatory T cells by antidiabetogenic MHC class II
AU - Tsai, Sue
AU - Serra, Paul
AU - Clemente-Casares, Xavier
AU - Slattery, Robyn Maree
AU - Santamaria, Pere
PY - 2013
Y1 - 2013
N2 - Several mechanisms have been proposed to explain how certain MHC class II molecules afford dominant resistance to autoimmune diseases like type 1 diabetes (T1D). However, it remains unclear how protective MHC types can blunt autoreactive T cell responses directed against a diverse repertoire of autoantigenic epitopes presented by disease-promoting MHCs. In this study, we show that expression of I-E on dendritic cells (DCs) of NOD mice promotes the differentiation of MHC promiscuous autoreactive CD4+ clonotypes into antidiabetogenic autoregulatory T cells. We expressed an I-EakloxP transgene in NOD mice and used cell type?specific I-E ablation to show that I-E?expressing DCs, but not B cells, promote the generation of autoreactive CD4+Foxp3+ regulatory T cells (Tregs) and their accumulation in the pancreas-draining lymph nodes. There, these Tregs suppress the presentation of ? cell Ags to naive autoreactive CD4+ and CD8+ T cells restricted by diabetogenic MHC molecules in an I-E?independent manner. Whereas selective removal of I-E on DCs abrogated autoregulatory Treg formation and T1D protection, selective removal of I-E on B cells was inconsequential. These results explain how certain MHC class II molecules can completely suppress antigenically complex autoimmune responses in an Ag-nonspecific manner.
AB - Several mechanisms have been proposed to explain how certain MHC class II molecules afford dominant resistance to autoimmune diseases like type 1 diabetes (T1D). However, it remains unclear how protective MHC types can blunt autoreactive T cell responses directed against a diverse repertoire of autoantigenic epitopes presented by disease-promoting MHCs. In this study, we show that expression of I-E on dendritic cells (DCs) of NOD mice promotes the differentiation of MHC promiscuous autoreactive CD4+ clonotypes into antidiabetogenic autoregulatory T cells. We expressed an I-EakloxP transgene in NOD mice and used cell type?specific I-E ablation to show that I-E?expressing DCs, but not B cells, promote the generation of autoreactive CD4+Foxp3+ regulatory T cells (Tregs) and their accumulation in the pancreas-draining lymph nodes. There, these Tregs suppress the presentation of ? cell Ags to naive autoreactive CD4+ and CD8+ T cells restricted by diabetogenic MHC molecules in an I-E?independent manner. Whereas selective removal of I-E on DCs abrogated autoregulatory Treg formation and T1D protection, selective removal of I-E on B cells was inconsequential. These results explain how certain MHC class II molecules can completely suppress antigenically complex autoimmune responses in an Ag-nonspecific manner.
UR - http://www.ncbi.nlm.nih.gov/pubmed/23740949
U2 - 10.4049/jimmunol.1300168
DO - 10.4049/jimmunol.1300168
M3 - Article
VL - 191
SP - 70
EP - 82
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 1
ER -