Delivery of non-toxic fragments of Clostridium difficile toxin a to the mucosal immune system

Stephen J. Ward, Gill Douce, Gordon Dougan, Brendan W. Wren

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Abstract

Polymerase chain reaction fragments coding for 8,14, 20 and 36 C-terminal repeats from Clostridhim difficile toxin A were expressed as fusions to fragment C of tetanus toxin. These fusions were expressed within a rationally attenuated Salmonella typhimurititn htrA mutant which has been shown to be effective at delivering heterologous antigens to the gastro-intestinal mucosa. The larger fragments retained their receptor-binding properties, and the construct containing 14 repeats induced an antitoxin A serum response within mice.

Original languageEnglish
JournalReviews in Medical Microbiology
Volume8
Publication statusPublished - 1 Dec 1997
Externally publishedYes

Keywords

  • Clostridhim difficile
  • Mucosal immunity
  • Toxin A
  • Vaccine

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