Polymerase chain reaction fragments coding for 8,14, 20 and 36 C-terminal repeats from Clostridhim difficile toxin A were expressed as fusions to fragment C of tetanus toxin. These fusions were expressed within a rationally attenuated Salmonella typhimurititn htrA mutant which has been shown to be effective at delivering heterologous antigens to the gastro-intestinal mucosa. The larger fragments retained their receptor-binding properties, and the construct containing 14 repeats induced an antitoxin A serum response within mice.
|Journal||Reviews in Medical Microbiology|
|Publication status||Published - 1 Dec 1997|
- Clostridhim difficile
- Mucosal immunity
- Toxin A