Abstract
The selection of αβ T cells in the thymus is punctuated by checkpoints at which thymocytes differentiate or undergo apoptosis. Wave 1 deletion is defined as apoptosis within nascent αβ T-cell antigen receptor (TCR)-signalled thymocytes that lack CCR7 expression. The antigen-presenting cell (APC) types that mediate wave 1 deletion are unclear. To measure wave 1 deletion, we compared the frequencies of TCRβ + CD5 + Helios + CCR7– cells in nascent thymocyte cohorts in mice with normal or defective apoptosis. This thymocyte population is small in mice lacking major histocompatibility complex (MHC) expression. The scale of wave 1 deletion was increased by transgenic expression of the self-reactive Yae62 TCRβ chain, was almost halved when haemopoietic APCs lacked MHC expression and, surprisingly, was unchanged when epithelial cells lacked MHC expression. These findings demonstrate efficiency, and some redundancy, in the APC types that mediate wave 1 deletion in the normal mouse thymus.
| Original language | English |
|---|---|
| Pages (from-to) | 2727-2739 |
| Number of pages | 13 |
| Journal | Cell Death and Differentiation |
| Volume | 26 |
| DOIs | |
| Publication status | Published - 24 Apr 2019 |
Keywords
- antigen-presenting cells
- immune cell death
- MHC
- T cells
Cite this
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Deletion of self-reactive CCR7– thymocytes in the absence of MHC expression on thymic epithelial cells. / Wirasinha, Rushika C.; Chan, Anna; Yap, Jin Yan; Hu, Daniel Y.; Teh, Charis E.; Gray, Daniel H.D.; Goodnow, Christopher C.; Daley, Stephen R.
In: Cell Death and Differentiation, Vol. 26, 24.04.2019, p. 2727-2739.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Deletion of self-reactive CCR7– thymocytes in the absence of MHC expression on thymic epithelial cells
AU - Wirasinha, Rushika C.
AU - Chan, Anna
AU - Yap, Jin Yan
AU - Hu, Daniel Y.
AU - Teh, Charis E.
AU - Gray, Daniel H.D.
AU - Goodnow, Christopher C.
AU - Daley, Stephen R.
PY - 2019/4/24
Y1 - 2019/4/24
N2 - The selection of αβ T cells in the thymus is punctuated by checkpoints at which thymocytes differentiate or undergo apoptosis. Wave 1 deletion is defined as apoptosis within nascent αβ T-cell antigen receptor (TCR)-signalled thymocytes that lack CCR7 expression. The antigen-presenting cell (APC) types that mediate wave 1 deletion are unclear. To measure wave 1 deletion, we compared the frequencies of TCRβ + CD5 + Helios + CCR7– cells in nascent thymocyte cohorts in mice with normal or defective apoptosis. This thymocyte population is small in mice lacking major histocompatibility complex (MHC) expression. The scale of wave 1 deletion was increased by transgenic expression of the self-reactive Yae62 TCRβ chain, was almost halved when haemopoietic APCs lacked MHC expression and, surprisingly, was unchanged when epithelial cells lacked MHC expression. These findings demonstrate efficiency, and some redundancy, in the APC types that mediate wave 1 deletion in the normal mouse thymus.
AB - The selection of αβ T cells in the thymus is punctuated by checkpoints at which thymocytes differentiate or undergo apoptosis. Wave 1 deletion is defined as apoptosis within nascent αβ T-cell antigen receptor (TCR)-signalled thymocytes that lack CCR7 expression. The antigen-presenting cell (APC) types that mediate wave 1 deletion are unclear. To measure wave 1 deletion, we compared the frequencies of TCRβ + CD5 + Helios + CCR7– cells in nascent thymocyte cohorts in mice with normal or defective apoptosis. This thymocyte population is small in mice lacking major histocompatibility complex (MHC) expression. The scale of wave 1 deletion was increased by transgenic expression of the self-reactive Yae62 TCRβ chain, was almost halved when haemopoietic APCs lacked MHC expression and, surprisingly, was unchanged when epithelial cells lacked MHC expression. These findings demonstrate efficiency, and some redundancy, in the APC types that mediate wave 1 deletion in the normal mouse thymus.
KW - antigen-presenting cells
KW - immune cell death
KW - MHC
KW - T cells
UR - http://www.scopus.com/inward/record.url?scp=85064746744&partnerID=8YFLogxK
U2 - 10.1038/s41418-019-0331-8
DO - 10.1038/s41418-019-0331-8
M3 - Article
VL - 26
SP - 2727
EP - 2739
JO - Cell Death and Differentiation
JF - Cell Death and Differentiation
SN - 1350-9047
ER -