Deleted in Colorectal Cancer (DCC) gene polymorphism is associated with H. pylori infection among susceptible Malays from the North-Eastern Region of Peninsular Malaysia

Sathiya Maran, Yeong Yeh Lee, Shuhua Xu, Nur Shafawati Rajab, Norhazrini Hasan, Nazri Mustaffa, Noorizan Abdul Majid, Zilfalil Bin Alwi

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7 Citations (Scopus)

Abstract

Background/Aims: Using genome-wide case-control association approach, the current study aimed to determine whether genetic polymorphism(s) is/are associated with H. pylori infection among ethnic Malays from the north-eastern region of Peninsular Malaysia, a region with an exceptionally low prevalence for H. pylori infection and gastric cancer. Methodology: Twenty-three Malay subjects positive for H. pylori confirmed with urease test and histology were enrolled as "cases" and 37 subjects negative for H. pylori were "controls". Both groups were matched for age and environmental risks. Extracted DNA samples (QIAGEN, Germany) from the venous blood of study subjects were genotyped using the Human Mapping 50k xba1 array (Affymetrix, USA). High throughput downstream analyses were then used to determine the significant SNP(s) associated with H. pylori infection. Results: Out of 20,361 SNPs filtered using the genotype association test, the top 1% (203) significant SNPs were selected for functional enrichment analysis. Of the 15 "enriched" SNPs, the rs10502974 which was located within the intronic region of Deleted in Colorectal Cancer (DCC) gene was the SNP most significantly associated with H. pylori infection (p=0.00549). Conclusions: Ethnic Malays is genetically susceptible to H. pylori infection and is possibly mediated through a genetic variation in the DCC gene.

Original languageEnglish
Pages (from-to)124-128
Number of pages5
JournalHepato-Gastroenterology
Volume60
Issue number122
DOIs
Publication statusPublished - Mar 2013
Externally publishedYes

Keywords

  • DCC gene
  • Genome wide association study
  • H. Pylori
  • Single nucleotide polymorphism

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