Deglycosylation of flavonoid and isoflavonoid glycosides by human small intestine and liver β-glucosidase activity

Andrea J. Day, M. Susan Dupont, Saxon Ridley, Mike Rhodes, Michael J.c. Rhodes, Michael R.a. Morgan, Gary Williamson

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Flavonoid and isoflavonoid glycosides are common dietary phenolics which may be absorbed from the small intestine of humans. The ability of cell-free extracts from human small intestine and liver to deglycosylate various (iso)flavonoid glycosides was investigated. Quercetin 4'-glucoside, naringenin 7-glucoside, apigenin 7-glucoside, genistein 7-glucoside and daidzein 7-glucoside were rapidly deglycosylated by both tissue extracts, whereas quercetin 3,4'-diglucoside, quercetin 3-glucoside, kaempferol 3-glucoside, quercetin 3-rhamnoglucoside and naringenin 7-rhamnoglucoside remained unchanged. The K(m) for hydrolysis of quercetin 4'-glucoside and genistein 7-glucoside was ~32±12 and ~14±3 μM in both tissues respectively. The enzymatic activity of the cell-free extracts exhibits similar properties to the cytosolic broad-specificity β-glucosidase previously described in mammals. Copyright (C) 1998 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)71-75
Number of pages5
JournalFEBS Letters
Issue number1
Publication statusPublished - 25 Sept 1998
Externally publishedYes


  • β-Glucosidase
  • Flavonoid
  • Flavonol
  • Genistein 7-glucoside
  • Isoflavonoid (human liver)
  • Quercetin 4'-glucoside

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