Defining the earliest step of cardiovascular progenitor specification during embryonic stem cell differentiation

Antoine Bondue, Simon Tännler, Giuseppe Chiapparo, Samira Chabab, Mirana Ramialison, Catherine Paulissen, Benjamin Beck, Richard P Harvey, Cédric Blanpain

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Abstract

During embryonic development and embryonic stem cell (ESC) differentiation, the different cell lineages of the mature heart arise from two types of multipotent cardiovascular progenitors (MCPs), the first and second heart fields. A key question is whether these two MCP populations arise from differentiation of a common progenitor. In this paper, we engineered Mesp1-green fluorescent protein (GFP) ESCs to isolate early MCPs during ESC differentiation. Mesp1-GFP cells are strongly enriched for MCPs, presenting the ability to differentiate into multiple cardiovascular lineages from both heart fields in vitro and in vivo. Transcriptional profiling of Mesp1-GFP cells uncovered cell surface markers expressed by MCPs allowing their prospective isolation. Mesp1 is required for MCP specification and the expression of key cardiovascular transcription factors. Isl1 is expressed in a subset of early Mesp1-expressing cells independently of Mesp1 and acts together with Mesp1 to promote cardiovascular differentiation. Our study identifies the early MCPs residing at the top of the cellular hierarchy of cardiovascular lineages during ESC differentiation. © 2011 Bondue et al.

Original languageEnglish
Pages (from-to)751-765
Number of pages15
JournalJournal of Cell Biology
Volume192
Issue number5
DOIs
Publication statusPublished - 7 Mar 2011
Externally publishedYes

Cite this

Bondue, A., Tännler, S., Chiapparo, G., Chabab, S., Ramialison, M., Paulissen, C., Beck, B., Harvey, R. P., & Blanpain, C. (2011). Defining the earliest step of cardiovascular progenitor specification during embryonic stem cell differentiation. Journal of Cell Biology, 192(5), 751-765. https://doi.org/10.1083/jcb.201007063