Defining membrane protein localization by isopycnic density gradients

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In many bacteria, membrane proteins account for around one-third of the proteome and can represent much more than half of the mass of a membrane. Classic techniques in cell biology can be applied to characterise bacterial membranes and their membrane protein constituents. Here we describe a protocol for the purification of outer and inner membranes from Escherichia coli. The procedure can be applied with minor modifications to other bacterial species, including those carrying capsular polysaccharide attached to the outer membrane.

Original languageEnglish
Title of host publicationBacterial Protein Secretion Systems
Subtitle of host publicationMethods and Protocols
EditorsLaure Journet, Eric Cascales
PublisherHumana Press
Number of pages6
ISBN (Electronic)9781493970339
ISBN (Print)9781493970315
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)10643745


  • Beta-barrel proteins
  • Cytoplasmic membrane
  • Lipoproteins
  • Membrane biogenesis
  • Sucrose density gradient

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