Defining membrane protein localization by isopycnic density gradients

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Abstract

In many bacteria, membrane proteins account for around one-third of the proteome and can represent much more than half of the mass of a membrane. Classic techniques in cell biology can be applied to characterise bacterial membranes and their membrane protein constituents. Here we describe a protocol for the purification of outer and inner membranes from Escherichia coli. The procedure can be applied with minor modifications to other bacterial species, including those carrying capsular polysaccharide attached to the outer membrane.

Original languageEnglish
Title of host publicationBacterial Protein Secretion Systems
Subtitle of host publicationMethods and Protocols
EditorsLaure Journet, Eric Cascales
PublisherHumana Press
Pages81-86
Number of pages6
Volume1615
ISBN (Electronic)978-1-4939-7033-9
ISBN (Print)978-1-4939-7031-5
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1615
ISSN (Print)10643745

Keywords

  • Beta-barrel proteins
  • Cytoplasmic membrane
  • Lipoproteins
  • Membrane biogenesis
  • Sucrose density gradient

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