@inbook{4f50b83cd99a4ee2a0c379c1d1bc2479,
title = "Defining membrane protein localization by isopycnic density gradients",
abstract = "In many bacteria, membrane proteins account for around one-third of the proteome and can represent much more than half of the mass of a membrane. Classic techniques in cell biology can be applied to characterise bacterial membranes and their membrane protein constituents. Here we describe a protocol for the purification of outer and inner membranes from Escherichia coli. The procedure can be applied with minor modifications to other bacterial species, including those carrying capsular polysaccharide attached to the outer membrane.",
keywords = "Beta-barrel proteins, Cytoplasmic membrane, Lipoproteins, Membrane biogenesis, Sucrose density gradient",
author = "Dunstan, {Rhys A} and Hay, {Iain D} and Trevor Lithgow",
year = "2017",
doi = "10.1007/978-1-4939-7033-9_6",
language = "English",
isbn = "978-1-4939-7031-5",
volume = "1615",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "81--86",
editor = "Laure Journet and Eric Cascales",
booktitle = "Bacterial Protein Secretion Systems",
address = "United States of America",
}