Deep sequencing identifies regulated small RNAs in Dugesia japonica

Zhenbiao Xu; Maoshan Chen; Zhonggan Ren; Nian Zhang; Hanmei Xu; Xiao Liu; Geng Tian; Linxia Song; Huanming Yang

doi:10.1007/s11033-012-2485-z

Deep sequencing identifies regulated small RNAs in Dugesia japonica

Zhenbiao Xu, Maoshan Chen, Zhonggan Ren, Nian Zhang, Hanmei Xu, Xiao Liu, Geng Tian, Linxia Song, Huanming Yang

Research output: Contribution to journal › Article › Research › peer-review

7 Citations (Scopus)

Abstract

MicroRNAs (miRNAs), which are 18 ∼ 24 nucleotides length, play important roles in regulating the expression of gene at the post-transcription level. Dugesia japonica is a branch of planarian organism. It is a model organism for studying the role of miRNAs in stem cell function. Next generation sequencing technology was used to identify the miRNAs of D. japonica. Bioinformatic analysis showed that 262 miRNA and miRNA*sequences were discovered, of which 102 miRNAs were the same as Schmidtea mediterranea and 160 miRNAs were related to other animals. There were 21 miRNAs expressed differentially after amputation. Results also revealed that some key miRNAs might play essential roles in the regeneration progress and some miRNAs might take part in the regulation progress of polarity regeneration in D. japonica.

Original language	English
Pages (from-to)	4075-4081
Number of pages	7
Journal	Molecular Biology Reports
Volume	40
Issue number	6
DOIs	https://doi.org/10.1007/s11033-012-2485-z
Publication status	Published - 1 Jun 2013
Externally published	Yes

Keywords

Deep sequencing
Dugesia japonica
MicroRNA
Planarian

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10.1007/s11033-012-2485-z

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title = "Deep sequencing identifies regulated small RNAs in Dugesia japonica",

abstract = "MicroRNAs (miRNAs), which are 18 ∼ 24 nucleotides length, play important roles in regulating the expression of gene at the post-transcription level. Dugesia japonica is a branch of planarian organism. It is a model organism for studying the role of miRNAs in stem cell function. Next generation sequencing technology was used to identify the miRNAs of D. japonica. Bioinformatic analysis showed that 262 miRNA and miRNA*sequences were discovered, of which 102 miRNAs were the same as Schmidtea mediterranea and 160 miRNAs were related to other animals. There were 21 miRNAs expressed differentially after amputation. Results also revealed that some key miRNAs might play essential roles in the regeneration progress and some miRNAs might take part in the regulation progress of polarity regeneration in D. japonica.",

keywords = "Deep sequencing, Dugesia japonica, MicroRNA, Planarian",

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T1 - Deep sequencing identifies regulated small RNAs in Dugesia japonica

AU - Xu, Zhenbiao

AU - Chen, Maoshan

AU - Ren, Zhonggan

AU - Zhang, Nian

AU - Xu, Hanmei

AU - Liu, Xiao

AU - Tian, Geng

AU - Song, Linxia

AU - Yang, Huanming

PY - 2013/6/1

Y1 - 2013/6/1

N2 - MicroRNAs (miRNAs), which are 18 ∼ 24 nucleotides length, play important roles in regulating the expression of gene at the post-transcription level. Dugesia japonica is a branch of planarian organism. It is a model organism for studying the role of miRNAs in stem cell function. Next generation sequencing technology was used to identify the miRNAs of D. japonica. Bioinformatic analysis showed that 262 miRNA and miRNA*sequences were discovered, of which 102 miRNAs were the same as Schmidtea mediterranea and 160 miRNAs were related to other animals. There were 21 miRNAs expressed differentially after amputation. Results also revealed that some key miRNAs might play essential roles in the regeneration progress and some miRNAs might take part in the regulation progress of polarity regeneration in D. japonica.

AB - MicroRNAs (miRNAs), which are 18 ∼ 24 nucleotides length, play important roles in regulating the expression of gene at the post-transcription level. Dugesia japonica is a branch of planarian organism. It is a model organism for studying the role of miRNAs in stem cell function. Next generation sequencing technology was used to identify the miRNAs of D. japonica. Bioinformatic analysis showed that 262 miRNA and miRNA*sequences were discovered, of which 102 miRNAs were the same as Schmidtea mediterranea and 160 miRNAs were related to other animals. There were 21 miRNAs expressed differentially after amputation. Results also revealed that some key miRNAs might play essential roles in the regeneration progress and some miRNAs might take part in the regulation progress of polarity regeneration in D. japonica.

KW - Deep sequencing

KW - Dugesia japonica

KW - MicroRNA

KW - Planarian

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Deep sequencing identifies regulated small RNAs in Dugesia japonica

Abstract

Keywords

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