Decreased phorbol ester binding in the parahippocampal gyrus from subjects with schizophrenia is not associated with changes in protein kinase C

E. Scarr, G. Pavey, P. J. Robinson, K. Opeskin, D. L. Copolov, B. Dean

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Abstract

Combining in situ radioligand binding with autoradiography, we previously identified a reduction of [3H]phorbol 12, 13-dibutyrate binding in the parahippocampal gyrus from schizophrenic subjects. To determine whether these changes were due to decreases in the level of protein kinase C, we measured [3H]phorbol 12, 13-dibutyrate binding, levels of the protein kinase C isoforms α, β, δ, ε, γ, η and θ, as well as protein kinase C activity in crude particulate membranes from parahippocampal gyri of 15 schizophrenic and 15 control subjects. There was a significant decrease in the density (mean ± SEM: 6.56 ± 0.73 pmol mg-1 vs 9.68 ± 1.22 pmol mg-1; P < 0.05) and affinity (mean KD ± SEM: 4.64 ± 0.34 nM vs 2.95 ± 0.35 nM; P < 0.005) of [3H]phorbol 12, 13-dibutyrate binding in homogenates from schizophrenic subjects. There were no significant changes in levels of the protein kinase C isoforms which are known to bind phorbol esters or in the activity of protein kinase C in membranes from schizophrenic subjects. These results suggest that there are changes in molecules capable of binding [3H]phorbol 12, 13-dibutyrate, other than protein kinase C, in the parahippocampal gyrus from subjects with schizophrenia.

Original languageEnglish
Pages (from-to)683-688
Number of pages6
JournalMolecular Psychiatry
Volume7
Issue number7
DOIs
Publication statusPublished - 1 Jan 2002
Externally publishedYes

Keywords

  • Human
  • Mental illness
  • Postmortem
  • Second messenger
  • Temporal lobe

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