@article{7d72dbab7ad84757a8038c9f97607946,
title = "Deciphering Hematopoietic Stem Cells in Their Niches: A Critical Appraisal of Genetic Models, Lineage Tracing, and Imaging Strategies",
abstract = "In recent years, technical developments in mouse genetics and imaging equipment have substantially advanced our understanding of hematopoietic stem cells (HSCs) and their niche. The availability of numerous Cre strains for targeting HSCs and microenvironmental cells provides extensive flexibility in experimental design, but it can also pose significant challenges due to strain-specific differences in cell specificity. Here we outline various genetic approaches for isolating, detecting, and ablating HSCs and niche components and provide a guide for advantages and caveats to consider. We also discuss opportunities and limitations presented by imaging technologies that allow investigation of HSC behavior in situ. A guide to the various genetic approaches and imaging technologies used to study HSCs and niche components is presented here.",
author = "Chacko Joseph and Quach, {Julie M.} and Walkley, {Carl R.} and Lane, {Steven W.} and {Lo Celso}, Cristina and Purton, {Louise E.}",
note = "Funding Information: We thank Dr. C. Maes for reading and commenting on the manuscript. This work was supported by grants from the National Health and Medical Research Council of Australia (NHMRC; to C.R.W., S.W.L., and L.E.P.); the Australian Cancer Research Foundation (to C.R.W. and L.E.P.); a NHMRC Senior Research Fellowship (L.E.P.); the Association for International Cancer Research (to L.E.P.); the Career Establishment Award from Cancer Research U.K. (to C.L.C.), and in part by the Victorian State Government Operational Infrastructure Support Program (to St. Vincent{\textquoteright}s Institute of Medical Research). C.R.W. is the Philip Desbrow Senior Research Fellow of the Leukaemia Foundation and S.W.L is a fellow of the Leukaemia Foundation. Publisher Copyright: {\textcopyright} 2013 Elsevier Inc.",
year = "2013",
month = nov,
day = "7",
doi = "10.1016/j.stem.2013.10.010",
language = "English",
volume = "13",
pages = "520--533",
journal = "Cell Stem Cell",
issn = "1934-5909",
publisher = "Elsevier",
number = "5",
}