Cytotoxic, anti-proliferative and apoptosis activity of l-amino acid oxidase from malaysian cryptelytrops purpureomaculatus (cp-laao) venom on human colon cancer cells

Syafiq Asnawi Zainal Abidin; Pathmanathan Rajadurai; Ezharul Hoque Chowdhury; Iekhsan Othman; Rakesh Naidu

doi:10.3390/molecules23061388

Cytotoxic, anti-proliferative and apoptosis activity of l-amino acid oxidase from malaysian cryptelytrops purpureomaculatus (cp-laao) venom on human colon cancer cells

Syafiq Asnawi Zainal Abidin, Pathmanathan Rajadurai, Ezharul Hoque Chowdhury, Iekhsan Othman, Rakesh Naidu

Malaysia Jeffrey Cheah Sch of Med & HS

Research output: Contribution to journal › Article › Research › peer-review

19 Citations (Scopus)

Abstract

The aim of this study is to investigate the potential anti-cancer activity of L-amino acid oxidase (CP-LAAO) purified from the venom of Cryptelytrops purpureomaculatus on SW480 and SW620 human colon cancer cells. Mass spectrometry guided purification was able to identify and purify CP-LAAO. Amino acid variations identified from the partial protein sequence of CP-LAAO may suggest novel variants of these proteins. The activity of the purified CP-LAAO was confirmed with o-phenyldiamine (OPD)-based spectrophotometric assay. CP-LAAO demonstrated time- and dose-dependent cytotoxic activity and the EC₅₀ value was determined at 13 µg/mL for both SW480 and SW620 cells. Significant increase of caspase-3 activity, reduction of Bcl-2 levels, as well as morphological changes consistent with apoptosis were demonstrated by CP-LAAO. Overall, these data provide evidence on the potential anti-cancer activity of CP-LAAO from the venom of Malaysian C. purpureomaculatus for therapeutic intervention of human colon cancer.

Original language	English
Article number	1388
Number of pages	16
Journal	Molecules
Volume	23
Issue number	6
DOIs	https://doi.org/10.3390/molecules23061388
Publication status	Published - 8 Jun 2018

Keywords

Apoptosis
Cryptelytrops purpureomaculatus
Cytotoxicity
Human colon cancer cells
L-amino acid oxidase

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/molecules23061388Licence: CC BY

337128974-oaFinal published version, 2.52 MBLicence: CC BY

Cite this

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title = "Cytotoxic, anti-proliferative and apoptosis activity of l-amino acid oxidase from malaysian cryptelytrops purpureomaculatus (cp-laao) venom on human colon cancer cells",

abstract = "The aim of this study is to investigate the potential anti-cancer activity of L-amino acid oxidase (CP-LAAO) purified from the venom of Cryptelytrops purpureomaculatus on SW480 and SW620 human colon cancer cells. Mass spectrometry guided purification was able to identify and purify CP-LAAO. Amino acid variations identified from the partial protein sequence of CP-LAAO may suggest novel variants of these proteins. The activity of the purified CP-LAAO was confirmed with o-phenyldiamine (OPD)-based spectrophotometric assay. CP-LAAO demonstrated time- and dose-dependent cytotoxic activity and the EC50 value was determined at 13 µg/mL for both SW480 and SW620 cells. Significant increase of caspase-3 activity, reduction of Bcl-2 levels, as well as morphological changes consistent with apoptosis were demonstrated by CP-LAAO. Overall, these data provide evidence on the potential anti-cancer activity of CP-LAAO from the venom of Malaysian C. purpureomaculatus for therapeutic intervention of human colon cancer.",

keywords = "Apoptosis, Cryptelytrops purpureomaculatus, Cytotoxicity, Human colon cancer cells, L-amino acid oxidase",

author = "Abidin, {Syafiq Asnawi Zainal} and Pathmanathan Rajadurai and Chowdhury, {Ezharul Hoque} and Iekhsan Othman and Rakesh Naidu",

year = "2018",

month = jun,

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language = "English",

volume = "23",

journal = "Molecules",

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Cytotoxic, anti-proliferative and apoptosis activity of l-amino acid oxidase from malaysian cryptelytrops purpureomaculatus (cp-laao) venom on human colon cancer cells. / Abidin, Syafiq Asnawi Zainal ; Rajadurai, Pathmanathan; Chowdhury, Ezharul Hoque et al.
In: Molecules, Vol. 23, No. 6, 1388, 08.06.2018.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Cytotoxic, anti-proliferative and apoptosis activity of l-amino acid oxidase from malaysian cryptelytrops purpureomaculatus (cp-laao) venom on human colon cancer cells

AU - Abidin, Syafiq Asnawi Zainal

AU - Rajadurai, Pathmanathan

AU - Chowdhury, Ezharul Hoque

AU - Othman, Iekhsan

AU - Naidu, Rakesh

PY - 2018/6/8

Y1 - 2018/6/8

N2 - The aim of this study is to investigate the potential anti-cancer activity of L-amino acid oxidase (CP-LAAO) purified from the venom of Cryptelytrops purpureomaculatus on SW480 and SW620 human colon cancer cells. Mass spectrometry guided purification was able to identify and purify CP-LAAO. Amino acid variations identified from the partial protein sequence of CP-LAAO may suggest novel variants of these proteins. The activity of the purified CP-LAAO was confirmed with o-phenyldiamine (OPD)-based spectrophotometric assay. CP-LAAO demonstrated time- and dose-dependent cytotoxic activity and the EC50 value was determined at 13 µg/mL for both SW480 and SW620 cells. Significant increase of caspase-3 activity, reduction of Bcl-2 levels, as well as morphological changes consistent with apoptosis were demonstrated by CP-LAAO. Overall, these data provide evidence on the potential anti-cancer activity of CP-LAAO from the venom of Malaysian C. purpureomaculatus for therapeutic intervention of human colon cancer.

AB - The aim of this study is to investigate the potential anti-cancer activity of L-amino acid oxidase (CP-LAAO) purified from the venom of Cryptelytrops purpureomaculatus on SW480 and SW620 human colon cancer cells. Mass spectrometry guided purification was able to identify and purify CP-LAAO. Amino acid variations identified from the partial protein sequence of CP-LAAO may suggest novel variants of these proteins. The activity of the purified CP-LAAO was confirmed with o-phenyldiamine (OPD)-based spectrophotometric assay. CP-LAAO demonstrated time- and dose-dependent cytotoxic activity and the EC50 value was determined at 13 µg/mL for both SW480 and SW620 cells. Significant increase of caspase-3 activity, reduction of Bcl-2 levels, as well as morphological changes consistent with apoptosis were demonstrated by CP-LAAO. Overall, these data provide evidence on the potential anti-cancer activity of CP-LAAO from the venom of Malaysian C. purpureomaculatus for therapeutic intervention of human colon cancer.

KW - Apoptosis

KW - Cryptelytrops purpureomaculatus

KW - Cytotoxicity

KW - Human colon cancer cells

KW - L-amino acid oxidase

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JO - Molecules

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Cytotoxic, anti-proliferative and apoptosis activity of l-amino acid oxidase from malaysian cryptelytrops purpureomaculatus (cp-laao) venom on human colon cancer cells

Abstract

Keywords

UN SDGs

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Potential human anti-colorectal cancer agents from Malaysian pit vipers

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Cytotoxic, anti-proliferative and apoptosis activity of l-amino acid oxidase from malaysian cryptelytrops purpureomaculatus (cp-laao) venom on human colon cancer cells

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Projects

Potential human anti-colorectal cancer agents from Malaysian pit vipers

Cite this