Abstract
To delineate the complexity of the response of cells to nanoparticles we have performed a study on HT-29 human colon carcinoma cells exposed first to a fullerene derivative C60(OH)20 and then to physiological copper ions. Our cell viability, proliferation, and intracellular reactive oxygen species (ROS) production assays clearly indicated that C 60(OH)20 suppressed cell damage as well as ROS production induced by copper, probably through neutralization of the metal ions by C 60(OH)20 in the extracellular space, as well as by adsorption and uptake of the nanoparticles surface-modified by the biomolecular species in the cell medium. This double-exposure study provides new data on the effects of nanoparticles on cell metabolism and may aid the treatment of oxidant-mediated diseases using nanomedicine.
Original language | English |
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Article number | 405101 |
Journal | Nanotechnology |
Volume | 22 |
Issue number | 40 |
DOIs | |
Publication status | Published - 7 Oct 2011 |
Externally published | Yes |