Cytokine removal with a large pore cellulose triacetate filter: An ex vivo study

S. Uchino, Rinaldo Bellomo, D. Goldsmith, P. Davenport, L. Cole, I. Baldwin, S. Panagiotopoulos, P. Tipping, C. Ronco, P. Everard

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Abstract

Objective: To test the hypothesis that hemofiltration using a new large pore cellulose triacetate hemofilter can achieve effective ultrafiltration of cytokines. Design: Ex-vivo study. Setting: Laboratory of Intensive Care Unit in tertiary hospital. Subjects: Six healthy volunteers. Interventions: Blood from 6 volunteers was incubated for 4 hours with 1 mg of endotoxin and then circulated through a closed hemofiltration circuit with a large pore cellulose triacetate hemofilter (nominal cut-off point: 60 kilodaltons). Hemofiltration was conducted at 1 L/h or 6 L/h of ultrafiltrate (UF) flow at the start of extra-corporeal circulation, and after 2 and 4 hours. Samples were taken from the arterial, venous and UF sampling ports. Measurements and main Results: IL-1β, IL-6, IL-8, IL-10, TNFα, and albumin were measured. Sieving coefficients (SC) above 0.6 were achieved for IL-1β and IL-6 and SCs above 0.3 were achieved for IL-8 and TNF-α at 1 L/h. Sieving coefficients of all cytokines (except IL-10, p=0.22) were reduced when the ultrafiltration rate was increased from IL/h to 6 L/h (p<0.01), but the increase in ultrafiltration rate resulted in an overall increase in the clearance of all cytokines (p<0.001). The highest SC for albumin was 0.07 at 4 hours at 1 L/h, and fell to 0.01 at 6 L/h. The SCs for IL-8 fell at 4 hours (p<0.01), but the SCs for other cytokines did not change. No adsorption of cytokines and albumin was observed. Conclusion: High volume hemofiltration (HVHF) using a new large pore cellulose triacetate filter achieved cytokine clearances greater than those reported with currently available hemofilters.

Original languageEnglish
Pages (from-to)27-32
Number of pages6
JournalInternational Journal of Artificial Organs
Volume25
Issue number1
Publication statusPublished - 27 Mar 2002
Externally publishedYes

Keywords

  • Acute renal failure
  • Blood purification
  • Cellulose triacetate
  • Critical illness
  • Cytokines
  • Hemofiltration
  • Interleukins
  • Sepsis

Cite this

Uchino, S., Bellomo, R., Goldsmith, D., Davenport, P., Cole, L., Baldwin, I., ... Everard, P. (2002). Cytokine removal with a large pore cellulose triacetate filter: An ex vivo study. International Journal of Artificial Organs, 25(1), 27-32.
Uchino, S. ; Bellomo, Rinaldo ; Goldsmith, D. ; Davenport, P. ; Cole, L. ; Baldwin, I. ; Panagiotopoulos, S. ; Tipping, P. ; Ronco, C. ; Everard, P. / Cytokine removal with a large pore cellulose triacetate filter : An ex vivo study. In: International Journal of Artificial Organs. 2002 ; Vol. 25, No. 1. pp. 27-32.
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abstract = "Objective: To test the hypothesis that hemofiltration using a new large pore cellulose triacetate hemofilter can achieve effective ultrafiltration of cytokines. Design: Ex-vivo study. Setting: Laboratory of Intensive Care Unit in tertiary hospital. Subjects: Six healthy volunteers. Interventions: Blood from 6 volunteers was incubated for 4 hours with 1 mg of endotoxin and then circulated through a closed hemofiltration circuit with a large pore cellulose triacetate hemofilter (nominal cut-off point: 60 kilodaltons). Hemofiltration was conducted at 1 L/h or 6 L/h of ultrafiltrate (UF) flow at the start of extra-corporeal circulation, and after 2 and 4 hours. Samples were taken from the arterial, venous and UF sampling ports. Measurements and main Results: IL-1β, IL-6, IL-8, IL-10, TNFα, and albumin were measured. Sieving coefficients (SC) above 0.6 were achieved for IL-1β and IL-6 and SCs above 0.3 were achieved for IL-8 and TNF-α at 1 L/h. Sieving coefficients of all cytokines (except IL-10, p=0.22) were reduced when the ultrafiltration rate was increased from IL/h to 6 L/h (p<0.01), but the increase in ultrafiltration rate resulted in an overall increase in the clearance of all cytokines (p<0.001). The highest SC for albumin was 0.07 at 4 hours at 1 L/h, and fell to 0.01 at 6 L/h. The SCs for IL-8 fell at 4 hours (p<0.01), but the SCs for other cytokines did not change. No adsorption of cytokines and albumin was observed. Conclusion: High volume hemofiltration (HVHF) using a new large pore cellulose triacetate filter achieved cytokine clearances greater than those reported with currently available hemofilters.",
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Uchino, S, Bellomo, R, Goldsmith, D, Davenport, P, Cole, L, Baldwin, I, Panagiotopoulos, S, Tipping, P, Ronco, C & Everard, P 2002, 'Cytokine removal with a large pore cellulose triacetate filter: An ex vivo study', International Journal of Artificial Organs, vol. 25, no. 1, pp. 27-32.

Cytokine removal with a large pore cellulose triacetate filter : An ex vivo study. / Uchino, S.; Bellomo, Rinaldo; Goldsmith, D.; Davenport, P.; Cole, L.; Baldwin, I.; Panagiotopoulos, S.; Tipping, P.; Ronco, C.; Everard, P.

In: International Journal of Artificial Organs, Vol. 25, No. 1, 27.03.2002, p. 27-32.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Cytokine removal with a large pore cellulose triacetate filter

T2 - An ex vivo study

AU - Uchino, S.

AU - Bellomo, Rinaldo

AU - Goldsmith, D.

AU - Davenport, P.

AU - Cole, L.

AU - Baldwin, I.

AU - Panagiotopoulos, S.

AU - Tipping, P.

AU - Ronco, C.

AU - Everard, P.

PY - 2002/3/27

Y1 - 2002/3/27

N2 - Objective: To test the hypothesis that hemofiltration using a new large pore cellulose triacetate hemofilter can achieve effective ultrafiltration of cytokines. Design: Ex-vivo study. Setting: Laboratory of Intensive Care Unit in tertiary hospital. Subjects: Six healthy volunteers. Interventions: Blood from 6 volunteers was incubated for 4 hours with 1 mg of endotoxin and then circulated through a closed hemofiltration circuit with a large pore cellulose triacetate hemofilter (nominal cut-off point: 60 kilodaltons). Hemofiltration was conducted at 1 L/h or 6 L/h of ultrafiltrate (UF) flow at the start of extra-corporeal circulation, and after 2 and 4 hours. Samples were taken from the arterial, venous and UF sampling ports. Measurements and main Results: IL-1β, IL-6, IL-8, IL-10, TNFα, and albumin were measured. Sieving coefficients (SC) above 0.6 were achieved for IL-1β and IL-6 and SCs above 0.3 were achieved for IL-8 and TNF-α at 1 L/h. Sieving coefficients of all cytokines (except IL-10, p=0.22) were reduced when the ultrafiltration rate was increased from IL/h to 6 L/h (p<0.01), but the increase in ultrafiltration rate resulted in an overall increase in the clearance of all cytokines (p<0.001). The highest SC for albumin was 0.07 at 4 hours at 1 L/h, and fell to 0.01 at 6 L/h. The SCs for IL-8 fell at 4 hours (p<0.01), but the SCs for other cytokines did not change. No adsorption of cytokines and albumin was observed. Conclusion: High volume hemofiltration (HVHF) using a new large pore cellulose triacetate filter achieved cytokine clearances greater than those reported with currently available hemofilters.

AB - Objective: To test the hypothesis that hemofiltration using a new large pore cellulose triacetate hemofilter can achieve effective ultrafiltration of cytokines. Design: Ex-vivo study. Setting: Laboratory of Intensive Care Unit in tertiary hospital. Subjects: Six healthy volunteers. Interventions: Blood from 6 volunteers was incubated for 4 hours with 1 mg of endotoxin and then circulated through a closed hemofiltration circuit with a large pore cellulose triacetate hemofilter (nominal cut-off point: 60 kilodaltons). Hemofiltration was conducted at 1 L/h or 6 L/h of ultrafiltrate (UF) flow at the start of extra-corporeal circulation, and after 2 and 4 hours. Samples were taken from the arterial, venous and UF sampling ports. Measurements and main Results: IL-1β, IL-6, IL-8, IL-10, TNFα, and albumin were measured. Sieving coefficients (SC) above 0.6 were achieved for IL-1β and IL-6 and SCs above 0.3 were achieved for IL-8 and TNF-α at 1 L/h. Sieving coefficients of all cytokines (except IL-10, p=0.22) were reduced when the ultrafiltration rate was increased from IL/h to 6 L/h (p<0.01), but the increase in ultrafiltration rate resulted in an overall increase in the clearance of all cytokines (p<0.001). The highest SC for albumin was 0.07 at 4 hours at 1 L/h, and fell to 0.01 at 6 L/h. The SCs for IL-8 fell at 4 hours (p<0.01), but the SCs for other cytokines did not change. No adsorption of cytokines and albumin was observed. Conclusion: High volume hemofiltration (HVHF) using a new large pore cellulose triacetate filter achieved cytokine clearances greater than those reported with currently available hemofilters.

KW - Acute renal failure

KW - Blood purification

KW - Cellulose triacetate

KW - Critical illness

KW - Cytokines

KW - Hemofiltration

KW - Interleukins

KW - Sepsis

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M3 - Article

C2 - 11853067

AN - SCOPUS:0036125413

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SP - 27

EP - 32

JO - International Journal of Artificial Organs

JF - International Journal of Artificial Organs

SN - 0391-3988

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Uchino S, Bellomo R, Goldsmith D, Davenport P, Cole L, Baldwin I et al. Cytokine removal with a large pore cellulose triacetate filter: An ex vivo study. International Journal of Artificial Organs. 2002 Mar 27;25(1):27-32.