TY - JOUR
T1 - CYP2E1 level in peripheral lymphocytes correlates with early pathogenesis of diabetic nephropathy
AU - Yap, Christina Gertrude
AU - Zaini Md Zain, Anuar
AU - Othman, Iekhsan
PY - 2013
Y1 - 2013
N2 - Since the goal of interventions for diabetic nephropathy is to prevent the progression of the disease, new early circulating biomolecules should be identified to be used as an early diagnostic tool instead of ?markers? of nephropathy in diabetes patients. Hence, it would be essential to identify the earliest possible biomolecules which could serve as an early predictor for hyperglycemia induced nephropathy. Reports have showed association of elevated CYP2E1 gene expression and protein level in peripheral lymphocytes with prolonged diabetes and chronic renal failure. Our objectives were to evaluate the correlation of lymphocyte CYP2E1 in diabetic nephropathy
and to identify other early peripheral biomolecules correlating with the onset of diabetic nephropathy. This is a cross-sectional cohort study, conducted on Malaysian subjects, consisted of control (C; n=28), type 2 diabetes (D; n=50), diabetic nephropathy (DN; n=34) and nondiabetic nephropathy (NDN; n=27) cohorts. Expression of CYP2E1 gene was evaluated using real-time PCR. Peripheral biomolecules correlating with onset of DN was investigated using microarray
analysis. Differential and correlation statistics were done using ANOVA on ranks test and Spearman Rank Order Correlation test respectively. P-values <0.05 were considered significant. Lymphocyte CYP2E1 levels correlated significantly with the increments of ACR and serum creatinine, and were detectable even when ACR and serum creatinine were still within normal reference limits. Further investigations are needed to determine the applicability of CYP2E1 as well as PGK1, GPI, ENO1, TPI1, ANGPTL4, AKR1B1 and SORD as candidate early risk predictors for diabetic nephropathy.
AB - Since the goal of interventions for diabetic nephropathy is to prevent the progression of the disease, new early circulating biomolecules should be identified to be used as an early diagnostic tool instead of ?markers? of nephropathy in diabetes patients. Hence, it would be essential to identify the earliest possible biomolecules which could serve as an early predictor for hyperglycemia induced nephropathy. Reports have showed association of elevated CYP2E1 gene expression and protein level in peripheral lymphocytes with prolonged diabetes and chronic renal failure. Our objectives were to evaluate the correlation of lymphocyte CYP2E1 in diabetic nephropathy
and to identify other early peripheral biomolecules correlating with the onset of diabetic nephropathy. This is a cross-sectional cohort study, conducted on Malaysian subjects, consisted of control (C; n=28), type 2 diabetes (D; n=50), diabetic nephropathy (DN; n=34) and nondiabetic nephropathy (NDN; n=27) cohorts. Expression of CYP2E1 gene was evaluated using real-time PCR. Peripheral biomolecules correlating with onset of DN was investigated using microarray
analysis. Differential and correlation statistics were done using ANOVA on ranks test and Spearman Rank Order Correlation test respectively. P-values <0.05 were considered significant. Lymphocyte CYP2E1 levels correlated significantly with the increments of ACR and serum creatinine, and were detectable even when ACR and serum creatinine were still within normal reference limits. Further investigations are needed to determine the applicability of CYP2E1 as well as PGK1, GPI, ENO1, TPI1, ANGPTL4, AKR1B1 and SORD as candidate early risk predictors for diabetic nephropathy.
UR - http://goo.gl/TrsxRa
M3 - Article
SN - 0970-938X
VL - 24
SP - 153
EP - 163
JO - Biomedical Research
JF - Biomedical Research
IS - 1
ER -