Cycloheximide can induce bax/bak dependent myeloid cell death independently of multiple BH3-only proteins

Katharine J. Goodall, Megan L. Finch-Edmondson, Joanne Van Vuuren, George Cheng Yeoh, Ian E Gentle, James E Vince, Paul G Ekert, David L. Vaux, Bernard A Callus

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

Apoptosis mediated by Bax or Bak is usually thought to be triggered by BH3-only members of the Bcl-2 protein family. BH3-only proteins can directly bind to and activate Bax or Bak, or indirectly activate them by binding to anti-apoptotic Bcl-2 family members, thereby relieving their inhibition of Bax and Bak. Here we describe a third way of activation of Bax/Bak dependent apoptosis that does not require triggering by multiple BH3-only proteins. In factor dependent myeloid (FDM) cell lines, cycloheximide induced apoptosis by a Bax/Bak dependent mechanism, because Bax-/- Bak-/- lines were profoundly resistant, whereas FDM lines lacking one or more genes for BH3-only proteins remained highly sensitive. Addition of cycloheximide led to the rapid loss of Mcl-1 but did not affect the expression of other Bcl-2 family proteins. In support of these findings, similar results were observed by treating FDM cells with the CDK inhibitor, roscovitine. Roscovitine reduced Mcl-1 abundance and caused Bax/Bak dependent cell death, yet FDM lines lacking one or more genes for BH3-only proteins remained highly sensitive. Therefore Bax/Bak dependent apoptosis can be regulated by the abundance of anti-apoptotic Bcl-2 family members such as Mcl-1, independently of several known BH3-only proteins.

Original languageEnglish
Article numbere0164003
Number of pages18
JournalPLoS ONE
Volume11
Issue number11
DOIs
Publication statusPublished - 2 Nov 2016
Externally publishedYes

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