Cyclin A2 modulates kinetochore-microtubule attachment in meiosis II

Qing-Hua Zhang, Wai Shan Yuen, Deepak Adhikari, Jennifer A. Flegg, Greg FitzHarris, Marco Conti, Piotr Sicinski, Ibtissem Nabti, Petros Marangos, John Carroll

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Cyclin A2 is a crucial mitotic Cdk regulatory partner that coordinates entry into mitosis and is then destroyed in prometaphase within minutes of nuclear envelope breakdown. The role of cyclin A2 in female meiosis and its dynamics during the transition from meiosis I (MI) to meiosis II (MII) remain unclear. We found that cyclin A2 decreases in prometaphase I but recovers after the first meiotic division and persists, uniquely for metaphase, in MII-arrested oocytes. Conditional deletion of cyclin A2 from mouse oocytes has no discernible effect on MI but leads to disrupted MII spindles and increased merotelic attachments. On stimulation of exit from MII, there is a dramatic increase in lagging chromosomes and an inhibition of cytokinesis. These defects are associated with an increase in microtubule stability in MII spindles, suggesting that cyclin A2 mediates the fidelity of MII by maintaining microtubule dynamics during the rapid formation of the MII spindle.

Original languageEnglish
Pages (from-to)3133-3143
Number of pages11
JournalJournal of Cell Biology
Volume216
Issue number10
DOIs
Publication statusPublished - 17 Aug 2017

Cite this

Zhang, Qing-Hua ; Yuen, Wai Shan ; Adhikari, Deepak ; Flegg, Jennifer A. ; FitzHarris, Greg ; Conti, Marco ; Sicinski, Piotr ; Nabti, Ibtissem ; Marangos, Petros ; Carroll, John. / Cyclin A2 modulates kinetochore-microtubule attachment in meiosis II. In: Journal of Cell Biology. 2017 ; Vol. 216, No. 10. pp. 3133-3143.
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abstract = "Cyclin A2 is a crucial mitotic Cdk regulatory partner that coordinates entry into mitosis and is then destroyed in prometaphase within minutes of nuclear envelope breakdown. The role of cyclin A2 in female meiosis and its dynamics during the transition from meiosis I (MI) to meiosis II (MII) remain unclear. We found that cyclin A2 decreases in prometaphase I but recovers after the first meiotic division and persists, uniquely for metaphase, in MII-arrested oocytes. Conditional deletion of cyclin A2 from mouse oocytes has no discernible effect on MI but leads to disrupted MII spindles and increased merotelic attachments. On stimulation of exit from MII, there is a dramatic increase in lagging chromosomes and an inhibition of cytokinesis. These defects are associated with an increase in microtubule stability in MII spindles, suggesting that cyclin A2 mediates the fidelity of MII by maintaining microtubule dynamics during the rapid formation of the MII spindle.",
author = "Qing-Hua Zhang and Yuen, {Wai Shan} and Deepak Adhikari and Flegg, {Jennifer A.} and Greg FitzHarris and Marco Conti and Piotr Sicinski and Ibtissem Nabti and Petros Marangos and John Carroll",
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Zhang, Q-H, Yuen, WS, Adhikari, D, Flegg, JA, FitzHarris, G, Conti, M, Sicinski, P, Nabti, I, Marangos, P & Carroll, J 2017, 'Cyclin A2 modulates kinetochore-microtubule attachment in meiosis II' Journal of Cell Biology, vol. 216, no. 10, pp. 3133-3143. https://doi.org/10.1083/jcb.201607111

Cyclin A2 modulates kinetochore-microtubule attachment in meiosis II. / Zhang, Qing-Hua; Yuen, Wai Shan; Adhikari, Deepak; Flegg, Jennifer A.; FitzHarris, Greg; Conti, Marco; Sicinski, Piotr; Nabti, Ibtissem; Marangos, Petros; Carroll, John.

In: Journal of Cell Biology, Vol. 216, No. 10, 17.08.2017, p. 3133-3143.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Cyclin A2 modulates kinetochore-microtubule attachment in meiosis II

AU - Zhang, Qing-Hua

AU - Yuen, Wai Shan

AU - Adhikari, Deepak

AU - Flegg, Jennifer A.

AU - FitzHarris, Greg

AU - Conti, Marco

AU - Sicinski, Piotr

AU - Nabti, Ibtissem

AU - Marangos, Petros

AU - Carroll, John

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