Cyclic nucleotide monophosphates in plants and plant signaling

Claudius Marondedze, Aloysius Wong, Ludivine Thomas, Helen Irving, Chris Gehring

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

Cyclic nucleotide monophosphates (cNMPs) and the enzymes that can generate them are of increasing interest in the plant sciences. Arguably, the major recent advance came with the release of the complete Arabidopsis thaliana genome that has enabled the systematic search for adenylate (ACs) or guanylate cyclases (GCs) and did eventually lead to the discovery of a number of GCs in higher plants. Many of these proteins have complex domain architectures with AC or GC centers moonlighting within cytosolic kinase domains. Recent reports indicated the presence of not just the canonical cNMPs (i.e., cAMP and cGMP), but also the noncanonical cCMP, cUMP, cIMP, and cdTMP in plant tissues, and this raises several questions. Firstly, what are the functions of these cNMPs, and, secondly, which enzymes can convert the substrate triphosphates into the respective noncanonical cNMPs? The first question is addressed here by comparing the reactive oxygen species (ROS) response of cAMP and cGMP to that elicited by the noncanonical cCMP or cIMP. The results show that particularly cIMP can induce significant ROS production. To answer, at least in part, the second question, we have evaluated homology models of experimentally confirmed plant GCs probing the substrate specificity by molecular docking simulations to determine if they can conceivably catalytically convert substrates other than ATP or GTP. In summary, molecular modeling and substrate docking simulations can contribute to the evaluation of cyclases for noncanonical cyclic mononucleotides and thereby further our understanding of the molecular mechanism that underlie cNMP-dependent signaling in planta.

Original languageEnglish
Title of host publicationNon-canonical Cyclic Nucleotides
EditorsRoland Seifert
Place of PublicationCham Switzerland
PublisherSpringer
Pages87-103
Number of pages17
Volume238
ISBN (Electronic)9783319526737
ISBN (Print)9783319526713
DOIs
Publication statusPublished - 2017

Publication series

NameHandbook of Experimental Pharmacology
Volume238
ISSN (Print)0171-2004
ISSN (Electronic)1865-0325

Keywords

  • Adenylate cyclase
  • Arabidopsis thaliana
  • cAMP
  • cGMP
  • Cyclic nucleotide monophosphates
  • Guanylate cyclase
  • Plant
  • Second messenger

Cite this

Marondedze, C., Wong, A., Thomas, L., Irving, H., & Gehring, C. (2017). Cyclic nucleotide monophosphates in plants and plant signaling. In R. Seifert (Ed.), Non-canonical Cyclic Nucleotides (Vol. 238, pp. 87-103). (Handbook of Experimental Pharmacology; Vol. 238). Cham Switzerland: Springer. https://doi.org/10.1007/164_2015_35
Marondedze, Claudius ; Wong, Aloysius ; Thomas, Ludivine ; Irving, Helen ; Gehring, Chris. / Cyclic nucleotide monophosphates in plants and plant signaling. Non-canonical Cyclic Nucleotides. editor / Roland Seifert. Vol. 238 Cham Switzerland : Springer, 2017. pp. 87-103 (Handbook of Experimental Pharmacology).
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Marondedze, C, Wong, A, Thomas, L, Irving, H & Gehring, C 2017, Cyclic nucleotide monophosphates in plants and plant signaling. in R Seifert (ed.), Non-canonical Cyclic Nucleotides. vol. 238, Handbook of Experimental Pharmacology, vol. 238, Springer, Cham Switzerland, pp. 87-103. https://doi.org/10.1007/164_2015_35

Cyclic nucleotide monophosphates in plants and plant signaling. / Marondedze, Claudius; Wong, Aloysius; Thomas, Ludivine; Irving, Helen; Gehring, Chris.

Non-canonical Cyclic Nucleotides. ed. / Roland Seifert. Vol. 238 Cham Switzerland : Springer, 2017. p. 87-103 (Handbook of Experimental Pharmacology; Vol. 238).

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

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AB - Cyclic nucleotide monophosphates (cNMPs) and the enzymes that can generate them are of increasing interest in the plant sciences. Arguably, the major recent advance came with the release of the complete Arabidopsis thaliana genome that has enabled the systematic search for adenylate (ACs) or guanylate cyclases (GCs) and did eventually lead to the discovery of a number of GCs in higher plants. Many of these proteins have complex domain architectures with AC or GC centers moonlighting within cytosolic kinase domains. Recent reports indicated the presence of not just the canonical cNMPs (i.e., cAMP and cGMP), but also the noncanonical cCMP, cUMP, cIMP, and cdTMP in plant tissues, and this raises several questions. Firstly, what are the functions of these cNMPs, and, secondly, which enzymes can convert the substrate triphosphates into the respective noncanonical cNMPs? The first question is addressed here by comparing the reactive oxygen species (ROS) response of cAMP and cGMP to that elicited by the noncanonical cCMP or cIMP. The results show that particularly cIMP can induce significant ROS production. To answer, at least in part, the second question, we have evaluated homology models of experimentally confirmed plant GCs probing the substrate specificity by molecular docking simulations to determine if they can conceivably catalytically convert substrates other than ATP or GTP. In summary, molecular modeling and substrate docking simulations can contribute to the evaluation of cyclases for noncanonical cyclic mononucleotides and thereby further our understanding of the molecular mechanism that underlie cNMP-dependent signaling in planta.

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Marondedze C, Wong A, Thomas L, Irving H, Gehring C. Cyclic nucleotide monophosphates in plants and plant signaling. In Seifert R, editor, Non-canonical Cyclic Nucleotides. Vol. 238. Cham Switzerland: Springer. 2017. p. 87-103. (Handbook of Experimental Pharmacology). https://doi.org/10.1007/164_2015_35