CX3CR1 reduces kidney fibrosis by inhibiting local proliferation of profibrotic macrophages

Daniel Robert Engel, Torsten A Krause, Sarah Louise Snelgrove, Stephanie Thiebes, Michael John Hickey, Peter Boor, Arthur Richard Kitching, Christian Kurts

Research output: Contribution to journalArticleResearchpeer-review

Abstract

A dense network of macrophages and dendritic cells (DC) expressing the chemokine receptor CX3CR1 populates most tissues. We recently reported that CX3CR1 regulates the abundance of CD11c(+) DC in the kidney and thereby promotes renal inflammation in glomerulonephritis. Given that chronic inflammation usually causes fibrosis, we hypothesized that CX3CR1 deficiency should attenuate renal fibrosis. However, when we tested this hypothesis using the DC-independent murine fibrosis model of unilateral ureteral obstruction, kidney fibrosis was unexpectedly more severe, despite less intrarenal inflammation. Two-photon imaging and flow cytometry revealed in kidneys of CX3CR1-deficient mice more motile Ly6C/Gr-1(+) macrophages. Flow cytometry verified that renal macrophages were more abundant in the absence of CX3CR1 and produced more of the key profibrotic mediator, TGF-beta. Macrophages accumulated because of higher intrarenal proliferation, despite reduced monocyte recruitment and higher signs of apoptosis within the kidney. These findings support the theory that tissue macrophage numbers are regulated through local proliferation and identify CX3CR1 as a regulator of such proliferation. Thus, CX3CR1 inhibition should be avoided in DC-independent inflammatory diseases because it may promote fibrosis.
Original languageEnglish
Pages (from-to)1628 - 1638
Number of pages11
JournalJournal of Immunology
Volume194
Issue number4
DOIs
Publication statusPublished - 2015

Cite this

Engel, Daniel Robert ; Krause, Torsten A ; Snelgrove, Sarah Louise ; Thiebes, Stephanie ; Hickey, Michael John ; Boor, Peter ; Kitching, Arthur Richard ; Kurts, Christian. / CX3CR1 reduces kidney fibrosis by inhibiting local proliferation of profibrotic macrophages. In: Journal of Immunology. 2015 ; Vol. 194, No. 4. pp. 1628 - 1638.
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abstract = "A dense network of macrophages and dendritic cells (DC) expressing the chemokine receptor CX3CR1 populates most tissues. We recently reported that CX3CR1 regulates the abundance of CD11c(+) DC in the kidney and thereby promotes renal inflammation in glomerulonephritis. Given that chronic inflammation usually causes fibrosis, we hypothesized that CX3CR1 deficiency should attenuate renal fibrosis. However, when we tested this hypothesis using the DC-independent murine fibrosis model of unilateral ureteral obstruction, kidney fibrosis was unexpectedly more severe, despite less intrarenal inflammation. Two-photon imaging and flow cytometry revealed in kidneys of CX3CR1-deficient mice more motile Ly6C/Gr-1(+) macrophages. Flow cytometry verified that renal macrophages were more abundant in the absence of CX3CR1 and produced more of the key profibrotic mediator, TGF-beta. Macrophages accumulated because of higher intrarenal proliferation, despite reduced monocyte recruitment and higher signs of apoptosis within the kidney. These findings support the theory that tissue macrophage numbers are regulated through local proliferation and identify CX3CR1 as a regulator of such proliferation. Thus, CX3CR1 inhibition should be avoided in DC-independent inflammatory diseases because it may promote fibrosis.",
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CX3CR1 reduces kidney fibrosis by inhibiting local proliferation of profibrotic macrophages. / Engel, Daniel Robert; Krause, Torsten A; Snelgrove, Sarah Louise; Thiebes, Stephanie; Hickey, Michael John; Boor, Peter; Kitching, Arthur Richard; Kurts, Christian.

In: Journal of Immunology, Vol. 194, No. 4, 2015, p. 1628 - 1638.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Krause, Torsten A

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AU - Boor, Peter

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AU - Kurts, Christian

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