TY - JOUR
T1 - Cutting edge
T2 - Generation of splenic CD8+ and CD8- dendritic cell equivalents in Fms-like tyrosine kinase 3 ligand bone marrow cultures
AU - Naik, Shalin H.
AU - Proietto, Anna I
AU - Wilson, Nicholas S.
AU - Dakic, Aleksandar
AU - Schnorrer, Petra
AU - Fuchsberger, Martina
AU - Lahoud, Mireille H.
AU - O'Keeffe, Meredith
AU - Shao, Qi Xiang
AU - Chen, Wei Feng
AU - Villadangos, José A.
AU - Shortman, Ken
AU - Wu, Li
PY - 2005/6/1
Y1 - 2005/6/1
N2 - We demonstrate that functional and phenotypic equivalents of mouse splenic CD8+ and CD8- conventional dendritic cell (cDC) subsets can be generated in vitro when bone marrow is cultured with fms-like tyrosine kinase 3 (flt3) ligand. In addition to CD45RAhigh plasmacytoid DC, two distinct CD24high and CD11bhigh cDC subsets were present, and these subsets showed equivalent properties to splenic CD8 + and CD8- cDC, respectively, in the following: 1) surface expression of CD11b, CD24, and signal regulatory protein-α; 2) developmental dependence on, and mRNA expression of, IFN regulatory factor-8; 3) mRNA expression of TLRs and chemokine receptors; 4) production of IL-12 p40/70, IFN-α, MIP-1α, and RANTES in response to TLR ligands; 5) expression of cystatin C; and 6) cross-presentation of exogenous Ag to CD8 T cells. Furthermore, despite lacking surface CD8 expression, the CD24high subset contained CD8 mRNA and up-regulated surface expression when transferred into mice. This culture system allows access to bonafide counterparts of the splenic DC subsets.
AB - We demonstrate that functional and phenotypic equivalents of mouse splenic CD8+ and CD8- conventional dendritic cell (cDC) subsets can be generated in vitro when bone marrow is cultured with fms-like tyrosine kinase 3 (flt3) ligand. In addition to CD45RAhigh plasmacytoid DC, two distinct CD24high and CD11bhigh cDC subsets were present, and these subsets showed equivalent properties to splenic CD8 + and CD8- cDC, respectively, in the following: 1) surface expression of CD11b, CD24, and signal regulatory protein-α; 2) developmental dependence on, and mRNA expression of, IFN regulatory factor-8; 3) mRNA expression of TLRs and chemokine receptors; 4) production of IL-12 p40/70, IFN-α, MIP-1α, and RANTES in response to TLR ligands; 5) expression of cystatin C; and 6) cross-presentation of exogenous Ag to CD8 T cells. Furthermore, despite lacking surface CD8 expression, the CD24high subset contained CD8 mRNA and up-regulated surface expression when transferred into mice. This culture system allows access to bonafide counterparts of the splenic DC subsets.
UR - http://www.scopus.com/inward/record.url?scp=21044458122&partnerID=8YFLogxK
M3 - Article
C2 - 15905497
AN - SCOPUS:21044458122
SN - 0022-1767
VL - 174
SP - 6592
EP - 6597
JO - Journal of Immunology
JF - Journal of Immunology
IS - 11
ER -