Current status of the Plasmodium falciparum genome project

John B. Dame, David E. Arnot, Peter F. Bourke, Debopam Chakrabarti, Zoe Christodoulou, Ross L. Coppel, Alan F. Cowman, Alister G. Craig, Katja Fischer, Jeremy Foster, Nathan Goodman, Katherine Hinterberg, Anthony A. Holder, Deborah C. Holt, David J. Kemp, Michael Lanzer, Amanda Lim, Chris I. Newbold, Jeffery V. Ravetch, G. Roman ReddyJustin Rubio, Sheldon M. Schuster, Xin Zhuan Su, Jennifer K. Thompson, Ferdinand Vital, Thomas E. Wellems, Ekkehard B. Werner

Research output: Contribution to journalShort SurveyOtherpeer-review

50 Citations (Scopus)

Abstract

The Plasmodium falciparum Genome Project is a collaborative effort by many laboratories that will provide detailed molecular information about the parasite, which may be used for developing practical control measures. Initial goals are to prepare an electronically indexed clone bank containing partially sequenced clones representing up to 80% of the parasite's genes and to prepare an ordered set of overlapping clones spanning each of the parasite's 14 chromosomes. Currently, clones of genomic DNA, prepared as yeast artificial chromosomes, are arranged into contigs covering ~ 70% of the genome of parasite clone 3D7, gene sequence tags are available from more than contigs covering ~ 70% of the genome of parasite clone 3D7, gene sequence tags are available from more than 20% of the parasite's genes, and ~ 5% of the parasite's genes are tentatively identified from similarity searches of entries in the international sequence databases. A total of > 0.5 Mb of P. falciparum sequence tag data is available. The gene sequence tags are presently being used to complete YAC contig assembly and localize the cloned genes to positions on the physical map in preparation for sequencing the genome. Routes of access to project information and services are described.

Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalMolecular and Biochemical Parasitology
Volume79
Issue number1
DOIs
Publication statusPublished - 1 Jan 1996

Keywords

  • cDNA
  • Expressed sequence tag
  • Genome project
  • Malaria
  • Mapping
  • Mung bean nuclease
  • Plasmodium falciparum
  • Protozoa
  • Review
  • Yeast artificial chromosome

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