Culturing of 'unculturable' human microbiota reveals novel taxa and extensive sporulation

Hilary P. Browne, Sam Forster, Blessing O. Anonye, Nitin Kumar, B. Anne Neville, Mark D. Stares, David Goulding, Trevor D. Lawley

Research output: Contribution to journalArticleResearchpeer-review

301 Citations (Scopus)

Abstract

Our intestinal microbiota harbours a diverse bacterial community required for our health, sustenance and wellbeing. Intestinal colonization begins at birth and climaxes with the acquisition of two dominant groups of strict anaerobic bacteria belonging to the Firmicutes and Bacteroidetes phyla. Culture-independent, genomic approaches have transformed our understanding of the role of the human microbiome in health and many diseases. However, owing to the prevailing perception that our indigenous bacteria are largely recalcitrant to culture, many of their functions and phenotypes remain unknown. Here we describe a novel workflow based on targeted phenotypic culturing linked to large-scale whole-genome sequencing, phylogenetic analysis and computational modelling that demonstrates that a substantial proportion of the intestinal bacteria are culturable. Applying this approach to healthy individuals, we isolated 137 bacterial species from characterized and candidate novel families, genera and species that were archived as pure cultures. Whole-genome and metagenomic sequencing, combined with computational and phenotypic analysis, suggests that at least 50-60% of the bacterial genera from the intestinal microbiota of a healthy individual produce resilient spores, specialized for host-to-host transmission. Our approach unlocks the human intestinal microbiota for phenotypic analysis and reveals how a marked proportion of oxygen-sensitive intestinal bacteria can be transmitted between individuals, affecting microbiota heritability.

Original languageEnglish
Pages (from-to)543-546
Number of pages4
JournalNature
Volume533
Issue number7604
DOIs
Publication statusPublished - 4 May 2016

Cite this

Browne, H. P., Forster, S., Anonye, B. O., Kumar, N., Neville, B. A., Stares, M. D., ... Lawley, T. D. (2016). Culturing of 'unculturable' human microbiota reveals novel taxa and extensive sporulation. Nature, 533(7604), 543-546. https://doi.org/10.1038/nature17645
Browne, Hilary P. ; Forster, Sam ; Anonye, Blessing O. ; Kumar, Nitin ; Neville, B. Anne ; Stares, Mark D. ; Goulding, David ; Lawley, Trevor D. / Culturing of 'unculturable' human microbiota reveals novel taxa and extensive sporulation. In: Nature. 2016 ; Vol. 533, No. 7604. pp. 543-546.
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Browne, HP, Forster, S, Anonye, BO, Kumar, N, Neville, BA, Stares, MD, Goulding, D & Lawley, TD 2016, 'Culturing of 'unculturable' human microbiota reveals novel taxa and extensive sporulation', Nature, vol. 533, no. 7604, pp. 543-546. https://doi.org/10.1038/nature17645

Culturing of 'unculturable' human microbiota reveals novel taxa and extensive sporulation. / Browne, Hilary P.; Forster, Sam; Anonye, Blessing O.; Kumar, Nitin; Neville, B. Anne; Stares, Mark D.; Goulding, David; Lawley, Trevor D.

In: Nature, Vol. 533, No. 7604, 04.05.2016, p. 543-546.

Research output: Contribution to journalArticleResearchpeer-review

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Browne HP, Forster S, Anonye BO, Kumar N, Neville BA, Stares MD et al. Culturing of 'unculturable' human microbiota reveals novel taxa and extensive sporulation. Nature. 2016 May 4;533(7604):543-546. https://doi.org/10.1038/nature17645