Culture of HIV in monocytes and macrophages

Suzanne Mary Crowe, Clare LV Maslin, Philip Ellery, Katherine Kedzierska

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Other

1 Citation (Scopus)

Abstract

Monocytes and macrophages constitute important cellular targets for macrophage-tropic (M-tropic) strains of HIV-1 and are believed to play a major role in the pathogenesis of AIDS. This updated unit provides procedures for culturing HIV in these target cells under suspension and adherent cell culture conditions. The unit also provides methods for expanding M-tropic primary isolates of HIV and determining the 50 tissue culture infectious dose (TCID50) of HIV stocks
Original languageEnglish
Title of host publicationCurrent Protocols in Immunology
EditorsJohn E Coligan, Barbara Bierer, David H Margulies, Ethan M Shevach, Warren Strober, Richard Coico
Place of PublicationUSA
PublisherJohn Wiley & Sons
Pages12.4.1 - 12.4.13
Number of pages13
ISBN (Print)1934-3671
DOIs
Publication statusPublished - 2004

Cite this