Culture of HIV in monocytes and macrophages

Suzanne Mary Crowe; Clare LV Maslin; Philip Ellery; Katherine Kedzierska

doi:10.1002/0471142735.im1204s62

Culture of HIV in monocytes and macrophages

Suzanne Mary Crowe, Clare LV Maslin, Philip Ellery, Katherine Kedzierska

Medicine Alfred Hospital

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other

1 Citation (Scopus)

Abstract

Monocytes and macrophages constitute important cellular targets for macrophage-tropic (M-tropic) strains of HIV-1 and are believed to play a major role in the pathogenesis of AIDS. This updated unit provides procedures for culturing HIV in these target cells under suspension and adherent cell culture conditions. The unit also provides methods for expanding M-tropic primary isolates of HIV and determining the 50 tissue culture infectious dose (TCID50) of HIV stocks

Original language	English
Title of host publication	Current Protocols in Immunology
Editors	John E Coligan, Barbara Bierer, David H Margulies, Ethan M Shevach, Warren Strober, Richard Coico
Place of Publication	USA
Publisher	John Wiley & Sons
Pages	12.4.1 - 12.4.13
Number of pages	13
ISBN (Print)	1934-3671
DOIs	https://doi.org/10.1002/0471142735.im1204s62
Publication status	Published - 2004

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10.1002/0471142735.im1204s62

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Cite this

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title = "Culture of HIV in monocytes and macrophages",

abstract = "Monocytes and macrophages constitute important cellular targets for macrophage-tropic (M-tropic) strains of HIV-1 and are believed to play a major role in the pathogenesis of AIDS. This updated unit provides procedures for culturing HIV in these target cells under suspension and adherent cell culture conditions. The unit also provides methods for expanding M-tropic primary isolates of HIV and determining the 50 tissue culture infectious dose (TCID50) of HIV stocks",

author = "Crowe, {Suzanne Mary} and Maslin, {Clare LV} and Philip Ellery and Katherine Kedzierska",

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booktitle = "Current Protocols in Immunology",

publisher = "John Wiley & Sons",

address = "United States of America",

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Culture of HIV in monocytes and macrophages. / Crowe, Suzanne Mary; Maslin, Clare LV; Ellery, Philip; Kedzierska, Katherine.

Current Protocols in Immunology. ed. / John E Coligan; Barbara Bierer; David H Margulies; Ethan M Shevach; Warren Strober; Richard Coico. USA : John Wiley & Sons, 2004. p. 12.4.1 - 12.4.13.

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other

TY - CHAP

T1 - Culture of HIV in monocytes and macrophages

AU - Crowe, Suzanne Mary

AU - Maslin, Clare LV

AU - Ellery, Philip

AU - Kedzierska, Katherine

PY - 2004

Y1 - 2004

N2 - Monocytes and macrophages constitute important cellular targets for macrophage-tropic (M-tropic) strains of HIV-1 and are believed to play a major role in the pathogenesis of AIDS. This updated unit provides procedures for culturing HIV in these target cells under suspension and adherent cell culture conditions. The unit also provides methods for expanding M-tropic primary isolates of HIV and determining the 50 tissue culture infectious dose (TCID50) of HIV stocks

AB - Monocytes and macrophages constitute important cellular targets for macrophage-tropic (M-tropic) strains of HIV-1 and are believed to play a major role in the pathogenesis of AIDS. This updated unit provides procedures for culturing HIV in these target cells under suspension and adherent cell culture conditions. The unit also provides methods for expanding M-tropic primary isolates of HIV and determining the 50 tissue culture infectious dose (TCID50) of HIV stocks

UR - http://mrw.interscience.wiley.com/emrw/9780471142737/cp/cpim/article/im1204/current/pdf

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DO - 10.1002/0471142735.im1204s62

M3 - Chapter (Book)

SN - 1934-3671

SP - 12.4.1 - 12.4.13

BT - Current Protocols in Immunology

A2 - Coligan, John E

A2 - Bierer, Barbara

A2 - Margulies, David H

A2 - Shevach, Ethan M

A2 - Strober, Warren

A2 - Coico, Richard

PB - John Wiley & Sons

CY - USA

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