Cultivation of Heligmosomoides polygyrus: An immunomodulatory nematode parasite and its secreted products

Chris J.C. Johnston; Elaine Robertson; Yvonne Harcus; John R. Grainger; Gillian Coakley; Danielle J. Smyth; Henry J. McSorley; Rick Maizels

doi:10.3791/52412

Cultivation of Heligmosomoides polygyrus: An immunomodulatory nematode parasite and its secreted products

Chris J.C. Johnston, Elaine Robertson, Yvonne Harcus, John R. Grainger, Gillian Coakley, Danielle J. Smyth, Henry J. McSorley, Rick Maizels

Research output: Contribution to journal › Article › Other › peer-review

69 Citations (Scopus)

Abstract

Heligmosomoides polygyrus (formerly known as Nematospiroides dubius, and also referred to by some as H. bakeri) is a gastrointestinal helminth that employs multiple immunomodulatory mechanisms to establish chronic infection in mice and closely resembles prevalent human helminth infections. H. polygyrus has been studied extensively in the field of helminth-derived immune regulation and has been found to potently suppress experimental models of allergy and autoimmunity (both with active infection and isolated secreted products). The protocol described in this paper outlines management of the H. polygyrus life cycle for consistent production of L3 larvae, recovery of adult parasites, and collection of their excretory-secretory products (HES).

Original language	English
Article number	e52412
Number of pages	10
Journal	Journal of Visualized Experiments
Volume	2015
Issue number	98
DOIs	https://doi.org/10.3791/52412
Publication status	Published - 6 Apr 2015
Externally published	Yes

Keywords

Excretory-Secretory products
Heligmosomoides polygyrus
Helminth
Immunology
Infection
Issue 98
Life cycle
Mouse

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10.3791/52412

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title = "Cultivation of Heligmosomoides polygyrus: An immunomodulatory nematode parasite and its secreted products",

abstract = "Heligmosomoides polygyrus (formerly known as Nematospiroides dubius, and also referred to by some as H. bakeri) is a gastrointestinal helminth that employs multiple immunomodulatory mechanisms to establish chronic infection in mice and closely resembles prevalent human helminth infections. H. polygyrus has been studied extensively in the field of helminth-derived immune regulation and has been found to potently suppress experimental models of allergy and autoimmunity (both with active infection and isolated secreted products). The protocol described in this paper outlines management of the H. polygyrus life cycle for consistent production of L3 larvae, recovery of adult parasites, and collection of their excretory-secretory products (HES).",

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T2 - An immunomodulatory nematode parasite and its secreted products

AU - Johnston, Chris J.C.

AU - Robertson, Elaine

AU - Harcus, Yvonne

AU - Grainger, John R.

AU - Coakley, Gillian

AU - Smyth, Danielle J.

AU - McSorley, Henry J.

AU - Maizels, Rick

PY - 2015/4/6

Y1 - 2015/4/6

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AB - Heligmosomoides polygyrus (formerly known as Nematospiroides dubius, and also referred to by some as H. bakeri) is a gastrointestinal helminth that employs multiple immunomodulatory mechanisms to establish chronic infection in mice and closely resembles prevalent human helminth infections. H. polygyrus has been studied extensively in the field of helminth-derived immune regulation and has been found to potently suppress experimental models of allergy and autoimmunity (both with active infection and isolated secreted products). The protocol described in this paper outlines management of the H. polygyrus life cycle for consistent production of L3 larvae, recovery of adult parasites, and collection of their excretory-secretory products (HES).

KW - Excretory-Secretory products

KW - Heligmosomoides polygyrus

KW - Helminth

KW - Immunology

KW - Infection

KW - Issue 98

KW - Life cycle

KW - Mouse

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ER -

Cultivation of Heligmosomoides polygyrus: An immunomodulatory nematode parasite and its secreted products

Abstract

Keywords

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