Crystallization of importin α, the nuclear-import receptor

Teh Trazel, Tony Tiganis, Bostjan Kobe

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20 Citations (Scopus)

Abstract

Crystals of recombinant importin α, the nuclear-import receptor, have been obtained at two different pH conditions by vapour diffusion using sodium citrate as precipitant and dithiothreitol as an additive. At pH 4-5, the crystals have the symmetry of the trigonal space group P3121 or P3221 (a = b = 78.0, c = 255.8 Å, γ = 120°); at pH 6-7, the crystals have the symmetry of the orthorhombic space group P212121 (a = 78.5, b = 89.7, c = 100.5 Å). In both cases, there is probably one molecule of importin α in the asymmetric unit. At least one of the crystal forms diffracts to a resolution higher than 3 Å using the laboratory X-ray source; the crystals are suitable for crystal structure determination.

Original languageEnglish
Pages (from-to)561-563
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume55
Issue number2
DOIs
Publication statusPublished - 1 Feb 1999
Externally publishedYes

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