Crystallization and preliminary X-ray analysis of the complex of the ε-subunit and the central domain of the γ-subunit from the Escherichia coli ATP synthase

A. Rodgers, G. Ewart, G. Cox, M. Wilce

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Abstract

A complex of the ε-subunit and the central domain of the γ-subunit from the ATP synthase of Escherichia coli has been purified and crystallized and preliminary X-ray analysis has been carried out. The crystals belong to space group C2221, with unit-cell parameters a = 76.7, b = 176.1, c = 67.1 Å (at 100 K). Determination of the structure of this protein complex promises to greatly improve the understanding of energy coupling between the F0 and F1 sectors within the enzyme complex.

Original languageEnglish
Pages (from-to)722-724
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume57
Issue number5
DOIs
Publication statusPublished - 21 May 2001
Externally publishedYes

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