Crystallization and preliminary x-ray analysis of the auto-inhibited twitchin kinase

Shu Hong Hu; Jun Yi Lei; Matthew C.J. Wilce; Mario R.L. Valenzuela; Guy M. Benian; Michael W. Parker; Bruce E. Kemp

doi:10.1016/0022-2836(94)90026-4

Crystallization and preliminary x-ray analysis of the auto-inhibited twitchin kinase

Shu Hong Hu, Jun Yi Lei, Matthew C.J. Wilce, Mario R.L. Valenzuela, Guy M. Benian, Michael W. Parker, Bruce E. Kemp

Research output: Contribution to journal › Article › Research › peer-review

7 Citations (Scopus)

Abstract

An auto-inhibited fragment of twitchin kinase (residues 5890 to 6262) has been crystallized by vapor diffusion techniques using polyethylene glycol 4000 as the precipitant at pH 7·25 to 7·5 at 4 °C. We have found that MgSO₄ and glycerol were essential for large crystal growth. The crystals belong to the orthorhombic space group P2₁2₁2, with unit cell dimensions of a = 144·1 A ̊, b = 168·3 A ̊ and c = 60·6 A ̊. They are suitable for X-ray analysis and diffract to a resolution of at least 2·8 Å.

Original language	English
Pages (from-to)	1259-1261
Number of pages	3
Journal	Journal of Molecular Biology
Volume	236
Issue number	4
DOIs	https://doi.org/10.1016/0022-2836(94)90026-4
Publication status	Published - 4 Mar 1994
Externally published	Yes

Keywords

auto-inhibition
crystals
pseudosubstrate
twitchin kinase
X-ray crystallography

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10.1016/0022-2836(94)90026-4

Cite this

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title = "Crystallization and preliminary x-ray analysis of the auto-inhibited twitchin kinase",

abstract = "An auto-inhibited fragment of twitchin kinase (residues 5890 to 6262) has been crystallized by vapor diffusion techniques using polyethylene glycol 4000 as the precipitant at pH 7·25 to 7·5 at 4 °C. We have found that MgSO4 and glycerol were essential for large crystal growth. The crystals belong to the orthorhombic space group P21212, with unit cell dimensions of a = 144·1 A ̊, b = 168·3 A ̊ and c = 60·6 A ̊. They are suitable for X-ray analysis and diffract to a resolution of at least 2·8 {\AA}.",

keywords = "auto-inhibition, crystals, pseudosubstrate, twitchin kinase, X-ray crystallography",

author = "Hu, \{Shu Hong\} and Lei, \{Jun Yi\} and Wilce, \{Matthew C.J.\} and Valenzuela, \{Mario R.L.\} and Benian, \{Guy M.\} and Parker, \{Michael W.\} and Kemp, \{Bruce E.\}",

year = "1994",

month = mar,

day = "4",

doi = "10.1016/0022-2836(94)90026-4",

language = "English",

volume = "236",

pages = "1259--1261",

journal = "Journal of Molecular Biology",

issn = "0022-2836",

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number = "4",

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T1 - Crystallization and preliminary x-ray analysis of the auto-inhibited twitchin kinase

AU - Hu, Shu Hong

AU - Lei, Jun Yi

AU - Wilce, Matthew C.J.

AU - Valenzuela, Mario R.L.

AU - Benian, Guy M.

AU - Parker, Michael W.

AU - Kemp, Bruce E.

PY - 1994/3/4

Y1 - 1994/3/4

N2 - An auto-inhibited fragment of twitchin kinase (residues 5890 to 6262) has been crystallized by vapor diffusion techniques using polyethylene glycol 4000 as the precipitant at pH 7·25 to 7·5 at 4 °C. We have found that MgSO4 and glycerol were essential for large crystal growth. The crystals belong to the orthorhombic space group P21212, with unit cell dimensions of a = 144·1 A ̊, b = 168·3 A ̊ and c = 60·6 A ̊. They are suitable for X-ray analysis and diffract to a resolution of at least 2·8 Å.

AB - An auto-inhibited fragment of twitchin kinase (residues 5890 to 6262) has been crystallized by vapor diffusion techniques using polyethylene glycol 4000 as the precipitant at pH 7·25 to 7·5 at 4 °C. We have found that MgSO4 and glycerol were essential for large crystal growth. The crystals belong to the orthorhombic space group P21212, with unit cell dimensions of a = 144·1 A ̊, b = 168·3 A ̊ and c = 60·6 A ̊. They are suitable for X-ray analysis and diffract to a resolution of at least 2·8 Å.

KW - auto-inhibition

KW - crystals

KW - pseudosubstrate

KW - twitchin kinase

KW - X-ray crystallography

UR - https://www.scopus.com/pages/publications/0028351418

U2 - 10.1016/0022-2836(94)90026-4

DO - 10.1016/0022-2836(94)90026-4

M3 - Article

C2 - 8120901

AN - SCOPUS:0028351418

SN - 0022-2836

VL - 236

SP - 1259

EP - 1261

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 4

ER -

Crystallization and preliminary x-ray analysis of the auto-inhibited twitchin kinase

Abstract

Keywords

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