Crystallization and initial X-ray data of abscisic acid receptor PYL3 in the presence of (-)-ABA

Xingliang Zhang, Qi Zhang, Guoqiang Wang

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Abscisic acid (ABA) modulates many complicated developmental processes and responses to environmental stimuli. Recently, several (+)-ABA signalling mechanisms by the RCAR/PYR1/PYL family of proteins (PYLs) have been proposed. However, the mechanism of the recognition and binding of the unnatural ligand (-)-ABA by PYLs has not yet been elucidated. In the present study, the expression, purification and crystallization of PYL3 in complex with (-)-ABA are reported. Diffraction data were refined to 2.65 Å resolution for this complex in space group P65. These findings will help to explain the stereospecificity of PYLs for (-)-ABA and to explore the selective ABA agonists.
Original languageEnglish
Pages (from-to)540-543
Number of pages4
JournalActa Crystallographica Section F: Structural Biology Communications
Volume69
Issue number5
DOIs
Publication statusPublished - May 2013
Externally publishedYes

Keywords

  • (-)-ABA
  • abscisic acid receptor
  • PYL3

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