Crystal structures of the human RNA demethylase alkbh5 reveal basis for substrate recognition

Chong Feng, Yang Liu, Guoqiang Wang, Zengqin Deng, Qi Zhang, Wei Wu, Yufeng Tong, Changmei Cheng, Zhongzhou Chen

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61 Citations (Scopus)

Abstract

Background: Human AlkB homolog 5 (Alkbh5) is an RNA demethylase that erases m6A modification.Results: Crystal structures of an enzymatically active Alkbh5 construct in complex with cofactors or small molecules were determined.
Conclusion: Structure and activity analyses showed that Alkbh5 strongly prefers single-stranded oligos and small molecule inhibitors.
Significance: The Alkbh5 structure reveals potential for structure-based design of selective inhibitors.
Original languageEnglish
Pages (from-to)11571-11583
Number of pages13
JournalThe Journal of Biological Chemistry
Volume289
Issue number17
DOIs
Publication statusPublished - 25 Apr 2014
Externally publishedYes

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