Cryogenic trapping as a versatile approach for sample handling, enrichment and multidimensional analysis in gas chromatography

Cryogenic methods – those that employ cryogenic fluids/gases but also other approaches to generate reduced temperature – are versatile, functional and relatively easily implemented as part of a total gas chromatographic method. The general utility of a cold region is almost invariably as a trapping or focussing step, to collect analyte into a sharp zone. The success in effectively trapping analyte depends on analyte volatility and the temperature of the cold region. Analytes collection into a sorbent phase supported by cryotrapping usually provide a greater capacity trapping for the sorption step. Stripping analyte from a sample into a cryogenic trap, with subsequent introduction to GC as in a purge-and-trap method, sample introduction into an injector with incorporation of a cooling zone, manipulation and management of chromatographic bands during chromatography elution such as employed in multidimensional gas chromatography, and focussing analyte just prior to the detector, all have the same goal of concentrating the band, reducing its dispersion, and maximising response. This review summarises various approaches that demonstrate how cryogenic methods have been incorporated into gas chromatographic analysis.