Cryo-scanning x-ray diffraction microscopy of frozen-hydrated yeast

E. Lima, A. Diaz, M. Guizar-Sicairos, S. Gorelick, P. Pernot, T. Schleier, A. Menzel

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40 Citations (Scopus)

Abstract

We developed cryo-scanning x-ray diffraction microscopy, utilizing hard x-ray ptychography at cryogenic temperature, for the noninvasive, high-resolution imaging of wet, extended biological samples and report its first frozen-hydrated imaging. Utilizing phase contrast at hard x-rays, cryo-scanning x-ray diffraction microscopy provides the penetration power suitable for thick samples while retaining sensitivity to minute density changes within unstained samples. It is dose-efficient and further minimizes radiation damage by keeping the wet samples at cryogenic temperature. We demonstrate these capabilities in two dimensions by imaging unstained frozen-hydrated budding yeast cells, achieving a spatial resolution of 85 nm with a phase sensitivity of 0.0053 radians. The current work presents the feasibility of cryo-scanning x-ray diffraction microscopy for quantitative, high-resolution imaging of unmodified biological samples extending to tens of micrometres.

Original languageEnglish
Number of pages7
JournalJournal of Microscopy
Volume249
Issue number1
DOIs
Publication statusPublished - 1 Jan 2013
Externally publishedYes

Keywords

  • Coherent x-ray diffraction
  • Frozen-hydrated imaging
  • Ptychography
  • Scanning x-ray diffraction microscopy

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