CrrB Positively Regulates High-Level Polymyxin Resistance and Virulence in Klebsiella pneumoniae

Thomas H. McConville; Medini K. Annavajhala; Marla J. Giddins; Nenad Macesic; Carmen M. Herrera; Felix D. Rozenberg; Gitanjali L. Bhushan; Danielle Ahn; Filippo Mancia; M. Stephen Trent; Anne Catrin Uhlemann

doi:10.1016/j.celrep.2020.108313

CrrB Positively Regulates High-Level Polymyxin Resistance and Virulence in Klebsiella pneumoniae

Thomas H. McConville
, Medini K. Annavajhala
, Marla J. Giddins
, Nenad Macesic
, Carmen M. Herrera
, Felix D. Rozenberg
, Gitanjali L. Bhushan
, Danielle Ahn
, Filippo Mancia
, M. Stephen Trent
, Anne Catrin Uhlemann

Infectious Diseases

Research output: Contribution to journal › Article › Research › peer-review

51 Citations (Scopus)

Abstract

McConville et al. leverage CRISPR-Cas to demonstrate that mutations in crrB induce high-level polymyxin resistance in Klebsiella pneumoniae via the addition of L-Ara4N and pEtN to lipid A. CrrB mutations also increase virulence while conferring a fitness cost and alter carbon metabolism through activation of the pentose phosphate pathway.

Original language	English
Article number	108313
Number of pages	14
Journal	Cell Reports
Volume	33
Issue number	4
DOIs	https://doi.org/10.1016/j.celrep.2020.108313
Publication status	Published - 27 Oct 2020

Keywords

antimicrobial resistance
colistin resistance
CRISPR-Cas9
CrrB
fitness
K. pneumoniae virulence
Klebsiella pneumoniae
polymyxin resistance

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10.1016/j.celrep.2020.108313Licence: CC BY-NC-ND

324905143-oaFinal published version, 2.19 MBLicence: CC BY-NC-ND

Cite this

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title = "CrrB Positively Regulates High-Level Polymyxin Resistance and Virulence in Klebsiella pneumoniae",

abstract = "McConville et al. leverage CRISPR-Cas to demonstrate that mutations in crrB induce high-level polymyxin resistance in Klebsiella pneumoniae via the addition of L-Ara4N and pEtN to lipid A. CrrB mutations also increase virulence while conferring a fitness cost and alter carbon metabolism through activation of the pentose phosphate pathway.",

keywords = "antimicrobial resistance, colistin resistance, CRISPR-Cas9, CrrB, fitness, K. pneumoniae virulence, Klebsiella pneumoniae, polymyxin resistance",

author = "McConville, \{Thomas H.\} and Annavajhala, \{Medini K.\} and Giddins, \{Marla J.\} and Nenad Macesic and Herrera, \{Carmen M.\} and Rozenberg, \{Felix D.\} and Bhushan, \{Gitanjali L.\} and Danielle Ahn and Filippo Mancia and Trent, \{M. Stephen\} and Uhlemann, \{Anne Catrin\}",

year = "2020",

month = oct,

day = "27",

doi = "10.1016/j.celrep.2020.108313",

language = "English",

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T1 - CrrB Positively Regulates High-Level Polymyxin Resistance and Virulence in Klebsiella pneumoniae

AU - McConville, Thomas H.

AU - Annavajhala, Medini K.

AU - Giddins, Marla J.

AU - Macesic, Nenad

AU - Herrera, Carmen M.

AU - Rozenberg, Felix D.

AU - Bhushan, Gitanjali L.

AU - Ahn, Danielle

AU - Mancia, Filippo

AU - Trent, M. Stephen

AU - Uhlemann, Anne Catrin

PY - 2020/10/27

Y1 - 2020/10/27

N2 - McConville et al. leverage CRISPR-Cas to demonstrate that mutations in crrB induce high-level polymyxin resistance in Klebsiella pneumoniae via the addition of L-Ara4N and pEtN to lipid A. CrrB mutations also increase virulence while conferring a fitness cost and alter carbon metabolism through activation of the pentose phosphate pathway.

AB - McConville et al. leverage CRISPR-Cas to demonstrate that mutations in crrB induce high-level polymyxin resistance in Klebsiella pneumoniae via the addition of L-Ara4N and pEtN to lipid A. CrrB mutations also increase virulence while conferring a fitness cost and alter carbon metabolism through activation of the pentose phosphate pathway.

KW - antimicrobial resistance

KW - colistin resistance

KW - CRISPR-Cas9

KW - CrrB

KW - fitness

KW - K. pneumoniae virulence

KW - Klebsiella pneumoniae

KW - polymyxin resistance

UR - https://www.scopus.com/pages/publications/85094562035

U2 - 10.1016/j.celrep.2020.108313

DO - 10.1016/j.celrep.2020.108313

M3 - Article

C2 - 33113377

AN - SCOPUS:85094562035

SN - 2211-1247

VL - 33

JO - Cell Reports

JF - Cell Reports

IS - 4

M1 - 108313

ER -

CrrB Positively Regulates High-Level Polymyxin Resistance and Virulence in Klebsiella pneumoniae

Abstract

Keywords

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