CrrB Positively Regulates High-Level Polymyxin Resistance and Virulence in Klebsiella pneumoniae

Thomas H. McConville; Medini K. Annavajhala; Marla J. Giddins; Nenad Macesic; Carmen M. Herrera; Felix D. Rozenberg; Gitanjali L. Bhushan; Danielle Ahn; Filippo Mancia; M. Stephen Trent; Anne Catrin Uhlemann

doi:10.1016/j.celrep.2020.108313

CrrB Positively Regulates High-Level Polymyxin Resistance and Virulence in Klebsiella pneumoniae

Thomas H. McConville, Medini K. Annavajhala, Marla J. Giddins, Nenad Macesic, Carmen M. Herrera, Felix D. Rozenberg, Gitanjali L. Bhushan, Danielle Ahn, Filippo Mancia, M. Stephen Trent, Anne Catrin Uhlemann

Infectious Diseases

Research output: Contribution to journal › Article › Research › peer-review

27 Citations (Scopus)

Abstract

McConville et al. leverage CRISPR-Cas to demonstrate that mutations in crrB induce high-level polymyxin resistance in Klebsiella pneumoniae via the addition of L-Ara4N and pEtN to lipid A. CrrB mutations also increase virulence while conferring a fitness cost and alter carbon metabolism through activation of the pentose phosphate pathway.

Original language	English
Article number	108313
Number of pages	14
Journal	Cell Reports
Volume	33
Issue number	4
DOIs	https://doi.org/10.1016/j.celrep.2020.108313
Publication status	Published - 27 Oct 2020

Keywords

antimicrobial resistance
colistin resistance
CRISPR-Cas9
CrrB
fitness
K. pneumoniae virulence
Klebsiella pneumoniae
polymyxin resistance

Access to Document

10.1016/j.celrep.2020.108313Licence: CC BY-NC-ND

324905143-oaFinal published version, 2.19 MBLicence: CC BY-NC-ND

Cite this

@article{f2a67c4283d4438d9cb2534a76a57f9f,

title = "CrrB Positively Regulates High-Level Polymyxin Resistance and Virulence in Klebsiella pneumoniae",

abstract = "McConville et al. leverage CRISPR-Cas to demonstrate that mutations in crrB induce high-level polymyxin resistance in Klebsiella pneumoniae via the addition of L-Ara4N and pEtN to lipid A. CrrB mutations also increase virulence while conferring a fitness cost and alter carbon metabolism through activation of the pentose phosphate pathway.",

keywords = "antimicrobial resistance, colistin resistance, CRISPR-Cas9, CrrB, fitness, K. pneumoniae virulence, Klebsiella pneumoniae, polymyxin resistance",

author = "McConville, {Thomas H.} and Annavajhala, {Medini K.} and Giddins, {Marla J.} and Nenad Macesic and Herrera, {Carmen M.} and Rozenberg, {Felix D.} and Bhushan, {Gitanjali L.} and Danielle Ahn and Filippo Mancia and Trent, {M. Stephen} and Uhlemann, {Anne Catrin}",

year = "2020",

month = oct,

day = "27",

doi = "10.1016/j.celrep.2020.108313",

language = "English",

volume = "33",

journal = "Cell Reports",

issn = "2211-1247",

publisher = "Elsevier",

number = "4",

}

TY - JOUR

T1 - CrrB Positively Regulates High-Level Polymyxin Resistance and Virulence in Klebsiella pneumoniae

AU - McConville, Thomas H.

AU - Annavajhala, Medini K.

AU - Giddins, Marla J.

AU - Macesic, Nenad

AU - Herrera, Carmen M.

AU - Rozenberg, Felix D.

AU - Bhushan, Gitanjali L.

AU - Ahn, Danielle

AU - Mancia, Filippo

AU - Trent, M. Stephen

AU - Uhlemann, Anne Catrin

PY - 2020/10/27

Y1 - 2020/10/27

N2 - McConville et al. leverage CRISPR-Cas to demonstrate that mutations in crrB induce high-level polymyxin resistance in Klebsiella pneumoniae via the addition of L-Ara4N and pEtN to lipid A. CrrB mutations also increase virulence while conferring a fitness cost and alter carbon metabolism through activation of the pentose phosphate pathway.

AB - McConville et al. leverage CRISPR-Cas to demonstrate that mutations in crrB induce high-level polymyxin resistance in Klebsiella pneumoniae via the addition of L-Ara4N and pEtN to lipid A. CrrB mutations also increase virulence while conferring a fitness cost and alter carbon metabolism through activation of the pentose phosphate pathway.

KW - antimicrobial resistance

KW - colistin resistance

KW - CRISPR-Cas9

KW - CrrB

KW - fitness

KW - K. pneumoniae virulence

KW - Klebsiella pneumoniae

KW - polymyxin resistance

UR - http://www.scopus.com/inward/record.url?scp=85094562035&partnerID=8YFLogxK

U2 - 10.1016/j.celrep.2020.108313

DO - 10.1016/j.celrep.2020.108313

M3 - Article

C2 - 33113377

AN - SCOPUS:85094562035

VL - 33

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 4

M1 - 108313

ER -

CrrB Positively Regulates High-Level Polymyxin Resistance and Virulence in Klebsiella pneumoniae

Abstract

Keywords

Access to Document

Other files and links

Cite this