CrrB Positively Regulates High-Level Polymyxin Resistance and Virulence in Klebsiella pneumoniae

Thomas H. McConville, Medini K. Annavajhala, Marla J. Giddins, Nenad Macesic, Carmen M. Herrera, Felix D. Rozenberg, Gitanjali L. Bhushan, Danielle Ahn, Filippo Mancia, M. Stephen Trent, Anne Catrin Uhlemann

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10 Citations (Scopus)

Abstract

McConville et al. leverage CRISPR-Cas to demonstrate that mutations in crrB induce high-level polymyxin resistance in Klebsiella pneumoniae via the addition of L-Ara4N and pEtN to lipid A. CrrB mutations also increase virulence while conferring a fitness cost and alter carbon metabolism through activation of the pentose phosphate pathway.

Original languageEnglish
Article number108313
Number of pages14
JournalCell Reports
Volume33
Issue number4
DOIs
Publication statusPublished - 27 Oct 2020

Keywords

  • antimicrobial resistance
  • colistin resistance
  • CRISPR-Cas9
  • CrrB
  • fitness
  • K. pneumoniae virulence
  • Klebsiella pneumoniae
  • polymyxin resistance

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