Cross-reactivity of a normal human cell surface antigen with primate retrovirus glycoproteins

Normal human cells express a human-specific antigen, HuLy-m5 (defined by the E4.3 monoclonal antibody), cross-reactive with determinants of the primate retroviruses, MPMV (Mason Pfizer monkey virus) and GALV (gibbon ape leukemia virus). Purified virus preparations of MPMV and GALV absorbed E4.3 antibody activity while antisera to these retroviruses blocked the binding of E4.3 anbitody to human target cells. Sequential immunoprecipitation and two-dimensional gel analysis both indicated that the anti-primate retrovirus sera recognize the same molecular entity (a two-chain glycoprotein of Mr 60 and 69Kd) as does the E4.3 antibody. These results suggest that normal human cells express primate retroviral proteins most probably viral envelope glycoprotein, gp69) at the cell surface.