Cross-platform compatibility of Hi-Plex, a streamlined approach for targeted massively parallel sequencing

Tú Nguyen-Dumont, Bernard J. Pope, Fleur Hammet, Maryam Mahmoodi, Helen Tsimiklis, Melissa C. Southey, Daniel J. Park

Research output: Contribution to journalArticleOtherpeer-review

9 Citations (Scopus)

Abstract

Although per-base sequencing costs have decreased during recent years, library preparation for targeted massively parallel sequencing remains constrained by high reagent cost, limited design flexibility, and protocol complexity. To address these limitations, we previously developed Hi-Plex, a polymerase chain reaction (PCR) massively parallel sequencing strategy for screening panels of genomic target regions. Here, we demonstrate that Hi-Plex applied with hybrid adapters can generate a library suitable for sequencing with both the Ion Torrent and the TruSeq chemistries and that adjusting primer concentrations improves coverage uniformity. These results expand Hi-Plex capabilities as an accurate, affordable, flexible, and rapid approach for various genetic screening applications.

Original languageEnglish
Pages (from-to)127-129
Number of pages3
JournalAnalytical Biochemistry
Volume442
Issue number2
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Disease gene screening
  • High-Plex PCR
  • Massively parallel sequencing
  • Molecular diagnostics
  • Targeted sequencing

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