TY - JOUR
T1 - Cross-complementation of Clostridium perfringens PLC and Clostridium septicum alpha-toxin mutants reveals PLC is sufficient to mediate gas gangrene
AU - Kennedy, Catherine Lydia
AU - Lyras, Dena
AU - Cheung, Kit-Ling Jackie
AU - Hiscox, Thomas Joseph
AU - Emmins, John Jeffrey
AU - Rood, Julian Ian
PY - 2009
Y1 - 2009
N2 - Clostridium perfringens and Clostridium septicum are the most common causes of clostridial myonecrosis or gas gangrene. Although they mediate a similar disease pathology, they elaborate functionally very different alpha-toxins. We used a reciprocal complementation approach to assess the contribution of the primary toxin of each species to disease and found that C. perfringens alpha-toxin (PLC) was able to mediate the gross pathology of myonecrosis even in a C. septicum background, although it could not induce vascular leukostasis. Conversely, while C. septicum alpha-toxin restored some virulence to a C. perfringens plc mutant, it was less active than in its native background.
AB - Clostridium perfringens and Clostridium septicum are the most common causes of clostridial myonecrosis or gas gangrene. Although they mediate a similar disease pathology, they elaborate functionally very different alpha-toxins. We used a reciprocal complementation approach to assess the contribution of the primary toxin of each species to disease and found that C. perfringens alpha-toxin (PLC) was able to mediate the gross pathology of myonecrosis even in a C. septicum background, although it could not induce vascular leukostasis. Conversely, while C. septicum alpha-toxin restored some virulence to a C. perfringens plc mutant, it was less active than in its native background.
UR - http://www.sciencedirect.com/science/article/pii/S1286457909000082
U2 - 10.1016/j.micinf.2009.01.002
DO - 10.1016/j.micinf.2009.01.002
M3 - Article
SN - 1286-4579
VL - 11
SP - 413
EP - 418
JO - Microbes and Infection
JF - Microbes and Infection
IS - 3
ER -