CRISPR/Cas9 in the chicken embryo

Valérie Morin, Nadège Véron, Christophe Marcelle

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

Genome editing is driving a revolution in the biomedical sciences that carries the promise for future treatments of genetic diseases. The CRISPR/Cas9 system of RNA-guided genome editing has been successfully applied to modify the genome of a wide spectrum of organisms. We recently showed that this technique can be combined with in vivo electroporation to inhibit the function of genes of interest in somatic cells of the developing chicken embryo. We present here a simplified version of the previously described technique that leads to effective gene loss-of-function.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages113-123
Number of pages11
Volume1650
DOIs
Publication statusPublished - 15 Aug 2017

Publication series

NameMethods in Molecular Biology
Volume1650
ISSN (Print)10643745

Keywords

  • Cas9
  • Chicken embryo
  • CRISPR
  • Electroporation
  • gRNA

Cite this

Morin, V., Véron, N., & Marcelle, C. (2017). CRISPR/Cas9 in the chicken embryo. In Methods in Molecular Biology (Vol. 1650, pp. 113-123). (Methods in Molecular Biology; Vol. 1650). Humana Press. https://doi.org/10.1007/978-1-4939-7216-6_7
Morin, Valérie ; Véron, Nadège ; Marcelle, Christophe. / CRISPR/Cas9 in the chicken embryo. Methods in Molecular Biology. Vol. 1650 Humana Press, 2017. pp. 113-123 (Methods in Molecular Biology).
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Morin, V, Véron, N & Marcelle, C 2017, CRISPR/Cas9 in the chicken embryo. in Methods in Molecular Biology. vol. 1650, Methods in Molecular Biology, vol. 1650, Humana Press, pp. 113-123. https://doi.org/10.1007/978-1-4939-7216-6_7

CRISPR/Cas9 in the chicken embryo. / Morin, Valérie; Véron, Nadège; Marcelle, Christophe.

Methods in Molecular Biology. Vol. 1650 Humana Press, 2017. p. 113-123 (Methods in Molecular Biology; Vol. 1650).

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

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Morin V, Véron N, Marcelle C. CRISPR/Cas9 in the chicken embryo. In Methods in Molecular Biology. Vol. 1650. Humana Press. 2017. p. 113-123. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7216-6_7