CRISPR/Cas9 in the chicken embryo

Valérie Morin, Nadège Véron, Christophe Marcelle

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

7 Citations (Scopus)

Abstract

Genome editing is driving a revolution in the biomedical sciences that carries the promise for future treatments of genetic diseases. The CRISPR/Cas9 system of RNA-guided genome editing has been successfully applied to modify the genome of a wide spectrum of organisms. We recently showed that this technique can be combined with in vivo electroporation to inhibit the function of genes of interest in somatic cells of the developing chicken embryo. We present here a simplified version of the previously described technique that leads to effective gene loss-of-function.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages113-123
Number of pages11
Volume1650
DOIs
Publication statusPublished - 15 Aug 2017

Publication series

NameMethods in Molecular Biology
Volume1650
ISSN (Print)10643745

Keywords

  • Cas9
  • Chicken embryo
  • CRISPR
  • Electroporation
  • gRNA

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