CRISPR mediated somatic cell genome engineering in the chicken

Nadege Veron, Zhengdong Qu, Phoebe A S Kipen, Claire E Hirst, Christophe Marcelle

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Gene-targeted knockout technologies are invaluable tools for understanding the functions of genes in vivo. CRISPR/Cas9 system of RNA-guided genome editing is revolutionizing genetics research in a wide spectrum of organisms. Here, we combined CRISPR with in vivo electroporation in the chicken embryo to efficiently target the transcription factor PAX7 in tissues of the developing embryo. This approach generated mosaic genetic mutations within a wild-type cellular background. This series of proof-of-principle experiments indicate that in vivo CRISPR-mediated cell genome engineering is an effective method to achieve gene loss-of-function in the tissues of the chicken embryo and it completes the growing genetic toolbox to study the molecular mechanisms regulating development in this important animal model.
Original languageEnglish
Pages (from-to)68 - 74
Number of pages7
JournalDevelopmental Biology
Volume407
Issue number1
DOIs
Publication statusPublished - 2015

Cite this

Veron, Nadege ; Qu, Zhengdong ; Kipen, Phoebe A S ; Hirst, Claire E ; Marcelle, Christophe. / CRISPR mediated somatic cell genome engineering in the chicken. In: Developmental Biology. 2015 ; Vol. 407, No. 1. pp. 68 - 74.
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CRISPR mediated somatic cell genome engineering in the chicken. / Veron, Nadege; Qu, Zhengdong; Kipen, Phoebe A S; Hirst, Claire E; Marcelle, Christophe.

In: Developmental Biology, Vol. 407, No. 1, 2015, p. 68 - 74.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - CRISPR mediated somatic cell genome engineering in the chicken

AU - Veron, Nadege

AU - Qu, Zhengdong

AU - Kipen, Phoebe A S

AU - Hirst, Claire E

AU - Marcelle, Christophe

PY - 2015

Y1 - 2015

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AB - Gene-targeted knockout technologies are invaluable tools for understanding the functions of genes in vivo. CRISPR/Cas9 system of RNA-guided genome editing is revolutionizing genetics research in a wide spectrum of organisms. Here, we combined CRISPR with in vivo electroporation in the chicken embryo to efficiently target the transcription factor PAX7 in tissues of the developing embryo. This approach generated mosaic genetic mutations within a wild-type cellular background. This series of proof-of-principle experiments indicate that in vivo CRISPR-mediated cell genome engineering is an effective method to achieve gene loss-of-function in the tissues of the chicken embryo and it completes the growing genetic toolbox to study the molecular mechanisms regulating development in this important animal model.

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