Projects per year
Abstract
Gene-targeted knockout technologies are invaluable tools for understanding the functions of genes in vivo. CRISPR/Cas9 system of RNA-guided genome editing is revolutionizing genetics research in a wide spectrum of organisms. Here, we combined CRISPR with in vivo electroporation in the chicken embryo to efficiently target the transcription factor PAX7 in tissues of the developing embryo. This approach generated mosaic genetic mutations within a wild-type cellular background. This series of proof-of-principle experiments indicate that in vivo CRISPR-mediated cell genome engineering is an effective method to achieve gene loss-of-function in the tissues of the chicken embryo and it completes the growing genetic toolbox to study the molecular mechanisms regulating development in this important animal model.
Original language | English |
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Pages (from-to) | 68 - 74 |
Number of pages | 7 |
Journal | Developmental Biology |
Volume | 407 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2015 |
Projects
- 4 Finished
-
Seeing is believing: imaging muscle maintenance and repair
Marcelle, C.
National Health and Medical Research Council (NHMRC) (Australia)
1/01/15 → 31/12/17
Project: Research
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Muscle fusion defects may be a common cause of human dystrophies
Marcelle, C.
National Health and Medical Research Council (NHMRC) (Australia)
1/01/14 → 31/12/16
Project: Research
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Cellular and molecular mechanisms underlying skeletal muscle organisation
Marcelle, C.
Australian Research Council (ARC)
3/01/13 → 31/12/15
Project: Research