Creating gradients of two proteins by differential passive adsorption onto a PEG-density gradient

Krasimir Vasilev, Agnieszka Mierczynska, Andrew L Hook, Joseph Chan, Nicolas H. Voelcker, Rob D. Short

Research output: Contribution to journalArticleResearchpeer-review

52 Citations (Scopus)

Abstract

Many fundamental biological processes, including early embryo development, immune responses and the progression of pathogens, are mediated by gradients of biological molecules. Understanding these vital physiological processes requires the development of biomaterial platforms that can mimic them in-vitro. Such platforms include laboratory generated surface gradients of biological molecules. In this work, we report a method for the generation of surface gradients of two proteins. We used a surface grafting density gradient of polyethylene glycol (PEG) to control protein adsorption. In addition, we used protein size as a tool to control the position and the adsorbed amount of both proteins. To demonstrate our concept, we used fibrinogen as an example of a large protein and lysozyme as an example of a small protein. However, we speculate that the same strategy could be extended to any other pair of large and small proteins. We used X-ray photoelectron spectroscopy and sessile drop contact angle measurements to determine the chemical composition and wettability of the gradients. Protein adsorption was studied by surface plasmon resonance imaging.

Original languageEnglish
Pages (from-to)392-397
Number of pages6
JournalBiomaterials
Volume31
Issue number3
DOIs
Publication statusPublished - Jan 2010
Externally publishedYes

Keywords

  • PEG gradient
  • Plasma deposition
  • Protein adsorption
  • Protein gradients
  • Surface grafting

Cite this