Cre recombinase specificity defined by the tau locus

Ella Korets-Smith, Lothar Lindemann, Kerry Lee Tucker, Caoyang Jiang, Nikolett Kabacs, Gusztav Belteki, Jody Haigh, Marina Gertsenstein, Andras Nagy

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15 Citations (Scopus)

Abstract

We generated a transgenic mouse line (tau::Cre) by targeting the Cre to the tau locus (Mapt). Based on previous reports on the expression of Tau during development, we expected the Cre recombinase to be expressed in a neuron-specific and pan-neuronal manner. However, intercrosses between the tau::Cre and the Cre-activatable reporter animals resulted in offspring with recombination either restricted to the nervous system or throughout the entire conceptus, indicating expression of Tau early in development. The percentage of neuron-specific excision was dependent on the Cre reporter used representing different Cre target sites in the mouse genome. In spite of the observed variability, our data suggest that the tau::Cre mouse line can be used for pan-neuronal recombination of floxed alleles when it is used with caution.
Original languageEnglish
Pages (from-to)131-138
Number of pages8
Journalgenesis: The Journal of Genetics and Development
Volume40
Issue number3
DOIs
Publication statusPublished - 2004
Externally publishedYes

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