TY - JOUR
T1 - Cre-loxP-Mediated Recombination
T2 - General Principles and Experimental Considerations
AU - McLellan, Micheal A.
AU - Rosenthal, Nadia A.
AU - Pinto, Alexander R.
PY - 2017/3/2
Y1 - 2017/3/2
N2 - The cre-loxP-mediated recombination system (the "cre-loxP system") is an integral experimental tool for mammalian genetics and cell biology. Use of the system has greatly expanded our ability to precisely interrogate gene function in the mouse, providing both spatial and temporal control of gene expression. This has been largely due to the simplicity of its use and its adaptability to address diverse biological questions. While the use of the cre-loxP system is becoming increasingly widespread, in particular because of growing availability of conditional mouse mutants, many considerations need to be taken into account when utilizing the cre-loxP system. This review provides an overview of the cre-loxP system and its various permutations. It addresses the limitations of cre-loxP technology and related considerations for experimental design, and it discusses alternative strategies for site-specific genetic recombination and integration.
AB - The cre-loxP-mediated recombination system (the "cre-loxP system") is an integral experimental tool for mammalian genetics and cell biology. Use of the system has greatly expanded our ability to precisely interrogate gene function in the mouse, providing both spatial and temporal control of gene expression. This has been largely due to the simplicity of its use and its adaptability to address diverse biological questions. While the use of the cre-loxP system is becoming increasingly widespread, in particular because of growing availability of conditional mouse mutants, many considerations need to be taken into account when utilizing the cre-loxP system. This review provides an overview of the cre-loxP system and its various permutations. It addresses the limitations of cre-loxP technology and related considerations for experimental design, and it discusses alternative strategies for site-specific genetic recombination and integration.
KW - conditional genetics
KW - cre
KW - cre-loxP
KW - gene editing
KW - loxP
KW - recombination
KW - site directed mutagenesis
UR - http://www.scopus.com/inward/record.url?scp=85015592755&partnerID=8YFLogxK
U2 - 10.1002/cpmo.22
DO - 10.1002/cpmo.22
M3 - Article
C2 - 28252198
AN - SCOPUS:85015592755
SN - 2161-2617
VL - 7
SP - 1
EP - 12
JO - Current Protocols in Mouse Biology
JF - Current Protocols in Mouse Biology
IS - 1
ER -