CpG pretreatment enhances antiviral T-cell immunity against cytomegalovirus

Monique Ong, Matthew E Wikstrom, Peter Fleming, Marie J Estcourt, Paul John Hertzog, Geoffrey R Hill, Christopher E Andoniou, Mariapia A Degli-Esposti

Research output: Contribution to journalArticleResearchpeer-review

10 Citations (Scopus)

Abstract

Major histocompatibility complex class I-restricted T-cell immunity is essential to control infection with cytomegalovirus (CMV), a clinically important virus that causes significant disease in immunocompromised individuals. Cross-presentation is considered the primary mode of antigen presentation to generate protective antiviral CD8+ T-cell immunity. Herpesviruses, including CMV, encode numerous proteins that interfere with direct antigen presentation, leading to the paradigm that T-cell immunity to these pathogens necessitates cross-presentation. However, the antigen presentation requirements needed to generate a protective T-cell response to CMV remain unknown. Here, we show that a fully functional antiviral CD8+ T-cell response can be generated in a system where cross-presentation is shut down by pretreatment with CpG. Notably, in this setting, CD8+ T cells demonstrate accelerated control of infection, and organ pathology is limited. These data indicate that protective antiviral T-cell immunity to CMV is generated by direct presentation and can be enhanced by pretreatment with CpG.
Original languageEnglish
Pages (from-to)55 - 60
Number of pages6
JournalBlood
Volume122
Issue number1
DOIs
Publication statusPublished - 2013

Cite this

Ong, Monique ; Wikstrom, Matthew E ; Fleming, Peter ; Estcourt, Marie J ; Hertzog, Paul John ; Hill, Geoffrey R ; Andoniou, Christopher E ; Degli-Esposti, Mariapia A. / CpG pretreatment enhances antiviral T-cell immunity against cytomegalovirus. In: Blood. 2013 ; Vol. 122, No. 1. pp. 55 - 60.
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abstract = "Major histocompatibility complex class I-restricted T-cell immunity is essential to control infection with cytomegalovirus (CMV), a clinically important virus that causes significant disease in immunocompromised individuals. Cross-presentation is considered the primary mode of antigen presentation to generate protective antiviral CD8+ T-cell immunity. Herpesviruses, including CMV, encode numerous proteins that interfere with direct antigen presentation, leading to the paradigm that T-cell immunity to these pathogens necessitates cross-presentation. However, the antigen presentation requirements needed to generate a protective T-cell response to CMV remain unknown. Here, we show that a fully functional antiviral CD8+ T-cell response can be generated in a system where cross-presentation is shut down by pretreatment with CpG. Notably, in this setting, CD8+ T cells demonstrate accelerated control of infection, and organ pathology is limited. These data indicate that protective antiviral T-cell immunity to CMV is generated by direct presentation and can be enhanced by pretreatment with CpG.",
author = "Monique Ong and Wikstrom, {Matthew E} and Peter Fleming and Estcourt, {Marie J} and Hertzog, {Paul John} and Hill, {Geoffrey R} and Andoniou, {Christopher E} and Degli-Esposti, {Mariapia A}",
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doi = "10.1182/blood-2012-12-471227",
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CpG pretreatment enhances antiviral T-cell immunity against cytomegalovirus. / Ong, Monique; Wikstrom, Matthew E; Fleming, Peter; Estcourt, Marie J; Hertzog, Paul John; Hill, Geoffrey R; Andoniou, Christopher E; Degli-Esposti, Mariapia A.

In: Blood, Vol. 122, No. 1, 2013, p. 55 - 60.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Ong, Monique

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AU - Fleming, Peter

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AU - Hill, Geoffrey R

AU - Andoniou, Christopher E

AU - Degli-Esposti, Mariapia A

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