TY - JOUR
T1 - CpG inhibits pro-B cell expansion through a cathepsin B-dependent mechanism
AU - Lalanne, Ana Inés
AU - Moraga, Ignacio
AU - Hao, Yi
AU - Pereira, João Pedro
AU - Alves, Nuno L.
AU - Huntington, Nicholas D.
AU - Freitas, Antonio A.
AU - Cumano, Ana
AU - Vieira, Paulo
PY - 2010/5/15
Y1 - 2010/5/15
N2 - TLR9 is expressed in cells of the innate immune system, as well as in B lymphocytes and their progenitors. We investigated the effect of the TLR9 ligand CpG DNA on the proliferation of pro-B cells. CpG DNA inhibits the proliferation of pro-B, but not pre-B, cells by inducing caspase-independent cell death through a pathway that requires the expression of cathepsin B. This pathway is operative in Rag-deficient mice carrying an SP6 transgene, in which B lymphopoiesis is compromised, to reduce the size of the B lymphocyte precursor compartments in the bone marrow. Thus, TLR9 signals can regulate B lymphopoiesis in vivo.
AB - TLR9 is expressed in cells of the innate immune system, as well as in B lymphocytes and their progenitors. We investigated the effect of the TLR9 ligand CpG DNA on the proliferation of pro-B cells. CpG DNA inhibits the proliferation of pro-B, but not pre-B, cells by inducing caspase-independent cell death through a pathway that requires the expression of cathepsin B. This pathway is operative in Rag-deficient mice carrying an SP6 transgene, in which B lymphopoiesis is compromised, to reduce the size of the B lymphocyte precursor compartments in the bone marrow. Thus, TLR9 signals can regulate B lymphopoiesis in vivo.
UR - http://www.scopus.com/inward/record.url?scp=77954714801&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.0903854
DO - 10.4049/jimmunol.0903854
M3 - Article
C2 - 20400700
AN - SCOPUS:77954714801
SN - 0022-1767
VL - 184
SP - 5678
EP - 5685
JO - Journal of Immunology
JF - Journal of Immunology
IS - 10
ER -