Corrigendum to: Online Pyrophosphate Assay for Analyzing Adenylation Domains of Nonribosomal Peptide Synthetases (ChemBioChem, (2016), 17, 7, (576-584), 10.1002/cbic.201500555)

Tiia Kittilä, Melanie Schoppet, Max J. Cryle

Research output: Contribution to journalComment / DebateOtherpeer-review

Abstract

When published, all enzyme assay components were commercially available. Recently, one essential component [fructose-6-phosphate kinase pyrophosphate-dependent (0.1 U mL-1; #F8381, Sigma–Aldrich)] has been discontinued. We direct readers to the following correspondence in which the purification of suitable replacement enzymes for inclusion in this assay is described by Machell et al.

Original languageEnglish
Article numbere202200381
Number of pages1
JournalChemBioChem
Volume23
Issue number18
DOIs
Publication statusPublished - 16 Sept 2022

Keywords

  • adenylation domain
  • antibiotics
  • enzymes
  • non-ribosomal peptide synthetase
  • pyrophosphate detection
  • teicoplanin

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